Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Tubercidin (Sparsomycin A) 是从Streptomyces tubercidicus 得到的抗生素,抑制粪链球菌生长的IC50值为 0.02 μM。它是腺苷磷酸化酶的弱抑制剂,并干扰腺苷和 AMP 的磷酸化。它有抗病毒作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 482 | 现货 | ||
10 mg | ¥ 753 | 现货 | ||
25 mg | ¥ 1,352 | 现货 | ||
50 mg | ¥ 1,997 | 现货 | ||
100 mg | ¥ 3,098 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 544 | 现货 |
产品描述 | Tubercidin (Sparsomycin A), an adenosine analogue, is a nucleoside antibiotic. It is incorporated into DNA and inhibits polymerases, thereby inhibiting DNA replication and RNA and protein synthesis. This agent also exhibits antifungal and antiviral activities. |
体外活性 | Continuous exposure for 14 days to tubercidin alone is highly toxic to both human CFU-GM and BFU-E. The IC50s of tubercidin are 3.4±1.7 and 3.7±0.2 nM for CFU-GM and BFU-E, respectively. Tubercidin also has a direct dose-dependent inhibitory effect on myeloid and erythroid human bone marrow progenitor cells in vitro[1]. |
体内活性 | Tubercidin at the dose regimen (5 mg/kg everyday for 4 days) is lethal when used alone. Some of the studies show that the mortality from tubercidin is primarily due to hepatic and renal injuries and, to a lesser extent, damage to the pancreas. Coadministration of NBMPR-P at 25 mg/kg per day completely protects the mice (100% survival) from this lethal dose regimen of tubercidin[1]. |
细胞实验 | Human bone marrow cells were obtained from healthy volunteers. In the protection studies, cells are exposed to 10 nM of tubercidin in the presence or absence of various doses of NBMPR-P. Cells are continuously exposed to drugs for 14 days in a humidified atmosphere of 5% C02-95% air at 37°C. Colonies of CFU-GM (250 cells) and BFU-E (>30 hemoglobinized cells) are counted with an inverted microscope. (Only for Reference) |
别名 | 结核菌素, 杀结核菌素, Sparsomycin A, 7-Deazaadenosine |
分子量 | 266.25 |
分子式 | C11H14N4O4 |
CAS No. | 69-33-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 49 mg/mL (184 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.7559 mL | 18.7793 mL | 37.5587 mL | 93.8967 mL |
5 mM | 0.7512 mL | 3.7559 mL | 7.5117 mL | 18.7793 mL | |
10 mM | 0.3756 mL | 1.8779 mL | 3.7559 mL | 9.3897 mL | |
20 mM | 0.1878 mL | 0.939 mL | 1.8779 mL | 4.6948 mL | |
50 mM | 0.0751 mL | 0.3756 mL | 0.7512 mL | 1.8779 mL | |
100 mM | 0.0376 mL | 0.1878 mL | 0.3756 mL | 0.939 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Tubercidin 69-33-0 Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology Influenza Virus DNA/RNA Synthesis Antibacterial Antibiotic Inhibitor 结核菌素 Bacterial 杀结核菌素 inhibit Sparsomycin A 7-Deazaadenosine inhibitor