Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Theaflavin 是在红茶中发现的一种多酚类黄酮,具有如抗氧化、抗癌、抗炎和抗病毒多种生物活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 283 | 现货 | ||
5 mg | ¥ 648 | 现货 | ||
10 mg | ¥ 950 | 现货 | ||
25 mg | ¥ 1,450 | 现货 | ||
50 mg | ¥ 2,150 | 现货 | ||
100 mg | ¥ 3,180 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 980 | 现货 |
产品描述 | Theaflavin is a polyphenolic flavonoid that has been found in black tea and has diverse biological activities, including antioxidant, anticancer, anti-inflammatory, and antiviral properties |
体外活性 | Theaflavin inhibits the growth of OVCAR-3 and A2780/CP70 human ovarian cancer cell lines, but not the ovarian epithelial cell line IOSE 364 (IC50s = 11.9, 38.5, and >40 μM, respectively)[1]. |
体内活性 | In the pretreated ICH rats TF significantly alleviated the behavioral defects, protected BBB integrity, and decreased the formation of cerebral edema and the levels of ROS as well as inflammatory cytokines (including interleukin-1 beta [IL-1β], IL-18, tumor nectosis factor-alpha, interferon-γ, transforming growth factor beta, and (C-X-C motif) ligand 1 [CXCL1]).?Nissl staining and TUNEL displayed TF could protect against the neuron loss and apoptosis via inhibiting the activation of nuclear transcription factor kappa-β-p65 (NF-κβ-p65), caspase-1, and IL-1β[2]. |
细胞实验 | Cell proliferation assay, Hoechst 33342 staining assay, Caspase-Glo Assay, western blot, human umbilical vein endothelial cell tube formation assay and vascular endothelial growth factor (VEGF) enzyme-linked immunosorbent assay were performed[1]. |
动物实验 | ICH rat models were induced with type VII collagenase and pretreated with TF by gavage in different doses (25 mg/kg-100 mg/kg).?Twenty-four hours after ICH attack, evaluated the rats' behavioral performance, the blood-brain barrier (BBB) integrity, and the formation of cerebral edema.?The levels of reactive oxygen species (ROS) and inflammatory cytokines were examined by 2',7'-dichlorofluorescin diacetate and enzyme-linked immunosorbent assay.?Nissl staining and transferase dUTP nick end labeling (TUNEL) were aimed to detect the neuron loss and apoptosis, the mechanism of which was explored by Western blot[2]. |
分子量 | 564.49 |
分子式 | C29H24O12 |
CAS No. | 4670-05-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 5.65 mg/mL (10 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.7715 mL | 8.8576 mL | 17.7151 mL | 44.2878 mL |
5 mM | 0.3543 mL | 1.7715 mL | 3.543 mL | 8.8576 mL | |
10 mM | 0.1772 mL | 0.8858 mL | 1.7715 mL | 4.4288 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Theaflavin 4670-05-7 Metabolism Microbiology/Virology Influenza Virus Endogenous Metabolite inhibit Inhibitor inhibitor