Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Terbutaline Sulfate (Terbutaline hemisulfate) 是一种具有口服活性的 β2-肾上腺素能受体激动剂,是班布特罗的活性代谢物。它可研究哮喘。
产品描述 | Terbutaline Sulfate (Terbutaline hemisulfate) is a selective beta-2 adrenergic agonist used as a bronchodilator and tocolytic. |
靶点活性 | β2-adrenoceptor:53 nM |
体外活性 | Terbutaline能够通过支气管,血管和子宫平滑肌的β2受体,产生刺激作用,也能够作用于子宫平滑肌,抑制子宫收缩. |
体内活性 | 在所有BChE变异体中,Terbutaline能够发挥可逆地竞争性的抑制作用,对于UU(Kd=0.18 mM),FF(Kd=0.31 mM)和AA纯合子(Kd=3.3 mM)。Terbutaline能够通过选择性作用于β2肾上腺素能发挥支气管扩张作用。 |
激酶实验 | Immunoprecipitation-HDAC assays: The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an 3H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [3H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C. |
别名 | Terbutaline hemisulfate, 硫酸叔丁宁按, 硫酸特布他林 |
分子量 | 274.32 |
分子式 | C12H19NO3·1/2H2SO4 |
CAS No. | 23031-32-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 5 mg/mL (18.23 mM), Sonification is recommended.
H2O: 100 mg/mL (364.54 mM), Sonification is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 3.6454 mL | 18.2269 mL | 36.4538 mL | 91.1344 mL |
5 mM | 0.7291 mL | 3.6454 mL | 7.2908 mL | 18.2269 mL | |
10 mM | 0.3645 mL | 1.8227 mL | 3.6454 mL | 9.1134 mL | |
H2O | 20 mM | 0.1823 mL | 0.9113 mL | 1.8227 mL | 4.5567 mL |
50 mM | 0.0729 mL | 0.3645 mL | 0.7291 mL | 1.8227 mL | |
100 mM | 0.0365 mL | 0.1823 mL | 0.3645 mL | 0.9113 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Terbutaline Sulfate 23031-32-5 GPCR/G Protein Microbiology/Virology Neuroscience Antibacterial Adrenergic Receptor Antibiotic macrophages Terbutaline Inhibitor J774 macrophages Beta Receptor Terbutaline Hemisulfate Terbutaline hemisulfate 硫酸叔丁宁按 asthma adrenergic receptor agonist MPK-1 inhibit 硫酸特布他林 inhibitor