Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Tebufenpyrad (Comanche) 是一种重要的农业化学杀螨剂,其作用类似于已知的线粒体毒物 rotenone。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 220 | 现货 | ||
100 mg | ¥ 750 | 现货 | ||
200 mg | ¥ 1,250 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 195 | 现货 |
产品描述 | Tebufenpyrad (Comanche) (mitochondrial complex-1 inhibitors) is an agro-chemically important acaricide that functions like the known mitochondrial toxicant rotenone. |
体外活性 | Exposing rat dopaminergic neuronal cells (N27 cells) to tebufenpyrad and pyridaben for 3 h induced dose-dependent cell death with an EC50 of 3.98 μM and 3.77 μM, respectively. Also, tebufenpyrad and pyridaben (3 μM) exposure induced reactive oxygen species (ROS) generation and m-aconitase damage, suggesting that the pesticide toxicity is associated with oxidative damage. Morphometric image analysis with the MitoTracker red fluorescent probe indicated that tebufenpyrad and pyridaben, as well as rotenone, caused abnormalities in mitochondrial morphology, including reduced mitochondrial length and circularity. Functional bioenergetic experiments using the Seahorse XF96 analyzer revealed that tebufenpyrad and pyridaben very rapidly suppressed the basal mitochondrial oxygen consumption rate similar to that of rotenone. Further analysis of bioenergetic curves also revealed dose-dependent decreases in ATP-linked respiration and respiratory capacity. The luminescence-based ATP measurement further confirmed that pesticide-induced mitochondrial inhibition of respiration is accompanied by the loss of cellular ATP [1]. |
别名 | Pyranica, 吡螨胺, MK239, Comanche |
分子量 | 333.86 |
分子式 | C18H24ClN3O |
CAS No. | 119168-77-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (179.72 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.9953 mL | 14.9763 mL | 29.9527 mL | 74.8817 mL |
5 mM | 0.5991 mL | 2.9953 mL | 5.9905 mL | 14.9763 mL | |
10 mM | 0.2995 mL | 1.4976 mL | 2.9953 mL | 7.4882 mL | |
20 mM | 0.1498 mL | 0.7488 mL | 1.4976 mL | 3.7441 mL | |
50 mM | 0.0599 mL | 0.2995 mL | 0.5991 mL | 1.4976 mL | |
100 mM | 0.03 mL | 0.1498 mL | 0.2995 mL | 0.7488 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Tebufenpyrad 119168-77-3 Metabolism Mitochondrial Metabolism Pyranica 吡螨胺 MK239 Comanche MK 239 MK-239 Inhibitor inhibitor inhibit