Powder: -20°C for 3 years | In solvent: -80°C for 1 year
TH34 是一种 HDAC6/8/10 抑制剂,IC50值分别为 4.6、1.9 和 7.7 μM。它显示出比 HDAC1/2/3 高的选择性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 328 | 现货 | ||
5 mg | ¥ 787 | 现货 | ||
10 mg | ¥ 1,160 | 现货 | ||
25 mg | ¥ 2,320 | 现货 | ||
50 mg | ¥ 3,480 | 现货 | ||
100 mg | ¥ 4,870 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 933 | 现货 |
产品描述 | TH34 is an HDAC6/8/10 inhibitor (IC50s: 4.6/1.9/7.7 μM). It shows high selectivity over HDAC1/2/3. |
靶点活性 | HDAC6:4.6 μM, HDAC8:1.9 μM, HDAC10:7.7 μM |
体外活性 | TH34 is well-tolerated by non-transformed human skin fibroblasts at concentrations up to 25 μM and modestly impairs colony growth in medulloblastoma cell lines, but specifically induces caspase-dependent programmed cell death in a concentration-dependent manner in several human neuroblastoma cell lines. |
细胞实验 | HeLa cells, stably transfected with NanoBRET plasmids NanoLuc?-HDAC6 FL Fusion Vector and NanoLuc?-HDAC10 FL Fusion Vector were seeded at 20,000 cells/well in white 96-well plates. Without further incubation, tracer (0.3 μM) and drugs were added in separate steps and plates were placed in a tissue culture incubator for 2 h. For NanoBRET quantification, plates were put at room temperature for 10 min. Nanoglow substrate, diluted in OptiMEM without phenol red, was added and measured within 10 min in an OPTIMA plate reader (460 nm emission for donor and 610LP filter for acceptor). The BRET signal was calculated by the ratio of acceptor signal to donor signal. |
分子量 | 256.3 |
分子式 | C15H16N2O2 |
CAS No. | 2196203-96-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: Insoluble
DMSO: 80 mg/mL (312.13 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.9017 mL | 19.5084 mL | 39.0168 mL | 97.5419 mL |
5 mM | 0.7803 mL | 3.9017 mL | 7.8034 mL | 19.5084 mL | |
10 mM | 0.3902 mL | 1.9508 mL | 3.9017 mL | 9.7542 mL | |
20 mM | 0.1951 mL | 0.9754 mL | 1.9508 mL | 4.8771 mL | |
50 mM | 0.078 mL | 0.3902 mL | 0.7803 mL | 1.9508 mL | |
100 mM | 0.039 mL | 0.1951 mL | 0.3902 mL | 0.9754 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
TH34 2196203-96-8 Chromatin/Epigenetic DNA Damage/DNA Repair HDAC TH 34 TH-34 inhibit Inhibitor Histone deacetylases inhibitor