Powder: -20°C for 3 years | In solvent: -80°C for 1 year
TCS 359 (FLT3 Inhibitor) 是 2-酰基氨基噻吩-3-甲酰胺,是选择性FLT3抑制剂(IC50:42 nM),能够抑制 MV4-11 细胞增殖(IC50:340 nM)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 498 | 现货 | ||
50 mg | ¥ 1,568 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 396 | 现货 |
产品描述 | TCS 359 (FLT3 Inhibitor) is a potent FLT3 inhibitor with IC50 of 42 nM. |
靶点活性 | FLT3:42 nM |
体外活性 | TCS 359, a 2-acylaminothiophene-3-carboxamide, is a potent inhibitor of FLT3 with IC50 of 42 nM. TCS 359 inhibits MV4-11 proliferation with IC50 of 340 nM. TCS 359 is highly selective for FLT3 against a panel of kinases. [1] |
激酶实验 | Affinity determination: To determine the activity of the compounds of the present invention in an in vitro kinase assay, inhibition of the isolated kinase domain of the human FLT3 receptor is performed using the following fluorescence polarization (FP) protocol. The FLT3 fluorescence polarization assay utilizes the fluorescein-labeled phosphopeptide and the anti-phosphotyrosine antibody included in the Panvera Phospho-Tyrosine Kinase Kit. The FLT3 kinase reaction is incubated at room temperature for 30 min under the following conditions: 10 nM FLT3 571-993, 20μg/mL poly Glu4Tyr, 150 μM ATP, 5 mM MgCl2, and 1% compound in DMSO. The kinase reaction is stopped with the addition of EDTA. The fluorescein-labeled phosphopeptide and the anti-phosphotyrosine antibody are added and incubated for 30 min at room temperature and polarization is read. |
细胞实验 | MV4-11 cells are plated at 10,000 cells per well in 100 μL of in RPMI media containing penn/strep, 10% FBS, and 0.2 ng/mL GM-CSF. Compound dilutions or 0.1% DMSO (vehicle control) is added to cells and the cells are allowed to grow for 72 h at standard cell growth conditions. To measure total cell growth, an equal volume of CellTiterGlo reagent is added to each well and luminescence is quantified. Total cell growth is quantified as the difference in luminescent counts of cell number at Day 0 compared to total cell number at Day 3 (72 h of growth and/or compound treatment). All IC50 values are calculated in GraphPadPrism using non-linear regression analysis with a multiparameter (variable slope) equation.(Only for Reference) |
别名 | FLT3 Inhibitor |
分子量 | 360.43 |
分子式 | C18H20N2O4S |
CAS No. | 301305-73-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 10.8 mg/mL (30 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.7745 mL | 13.8723 mL | 27.7446 mL | 69.3616 mL |
5 mM | 0.5549 mL | 2.7745 mL | 5.5489 mL | 13.8723 mL | |
10 mM | 0.2774 mL | 1.3872 mL | 2.7745 mL | 6.9362 mL | |
20 mM | 0.1387 mL | 0.6936 mL | 1.3872 mL | 3.4681 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
TCS 359 301305-73-7 Angiogenesis Tyrosine Kinase/Adaptors FLT FLT3 inhibit Fms like tyrosine kinase 3 TCS-359 TCS359 Cluster of differentiation antigen 135 Inhibitor CD135 FLT3 Inhibitor inhibitor