Sulforaphane

产品编号: TQ0207 别名:

油菜,萝卜硫素

Sulforaphane 是存在于多种蔬菜中的一种天然异硫氰酸酯。它可以激活Nrf2，并通过 AMPK 依赖性信号传导抑制高糖诱导的胰腺癌。它增加肿瘤抑制蛋白的转录并抑制组蛋白脱乙酰酶的活性，具有抗癌和抗炎活性。

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CAS：4478-93-7

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生物活性化学信息储存和溶解度信息参考文献引用文献

产品描述

Sulforaphane is derived from the consumption of cruciferous vegetables, such as broccoli. It has shown anticancer and cardioprotective activities.

体外活性

Sulforaphane induces a cell cycle arrest in a dose-dependent manner, followed by cell death. This sulforaphane-induced cell cycle arrest was correlated with an increased expression of cyclins A and B1. Sulforaphane induces cell death via an apoptotic process. Sulforaphane inhibits the reinitiation of growth and diminishes cellular viability in quiescent colon carcinoma cells (HT29) and has lower toxicity on differentiated CaCo2 cells [1]. Pre-treatment of H9c2 rat myoblasts with sulforaphane decreases the apoptotic cell number and the expression of pro-apoptotic proteins, as well as the doxorubicin-induced increase in mitochondrial membrane potential. Furthermore, sulforaphane increases the mRNA and protein expression of heme oxygenase-1, which consequently reduces the levels of reactive oxygen species in the mitochondria which are induced by doxorubicin [2].

体内活性

Administration of sulforaphane reduces the incidence, multiplicity, and weights and delays the development of the mammary tumors evoked by a single dose of DMBA in female SD rats [3].

细胞实验

HT29 cells are seeded at low density (5×10^4 cells/mL) in 35- or 120-mm diameter Primaria dishes in standard medium containing 5% FCS. One day after seeding, the medium is changed, and HT29 cells are treated with sulforaphane (0-30 μM). An equivalent amount of the solvent (ethanol) is added to control cells (0.2% final concentration). The drug effect on cellular viability is evaluated using the MTT assay [1].

动物实验

At age 47, 48, 49, 50, and 51 days, each animal receives by gavage either 0.5 mL of Emulphor EL-620 alone or the specified doses (75, 100, or 150 μM daily) of sulforaphane or compound 2, 3, or 4 in 0.5 mL of Emulphor EL-620. On day 50, 3 hr after administration of the vehicle or protector, all rats also receive an intragastric instillation of 8.0 mg of DMBA dissolved in 1.0 mL of sesame oil. This dose of DMBA is selected to produce a substantial tumor incidence, but not one so high as to overwhelm a potential chemoprotective effect. The animals are examined once weekly for the appearance and location of palpable tumors. At age 202 days, i.e., 152 days after carcinogen administration, all animals are euthanized with ether and weighed. The tumors are separated from the fat and connective tissue by dissection weighed and fixed in buffered 10% formalin. All tumors are identified microscopically by examination of stained sections [3].

Cas No.

4478-93-7

分子式

C6H11NOS2

分子量

177.29

别名

油菜;萝卜硫素;Sulforaphane

参考文献

[1]Gamet-Payrastre L, et al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res. 2000 Mar 1;60(5):1426-33.[2]Li B, et al. Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells. Int J Mol Med. 2015 Jul;36(1):53-64.[3]Zhang Y, et al. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornylisothiocyanates. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3147-50.

储存和溶解度

DMSO:60 mg/mL (338.43 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years

剂量换算 Sulforaphane 4478-93-7

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Keywords

Inhibitor Keap1-Nrf2 Apoptosis Sulforaphane Histone deacetylases inhibit 油菜萝卜硫素 HDAC 4478-93-7

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Sulforaphane

油菜,萝卜硫素

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