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Sulforaphane

Sulforaphane

产品编号 TQ0207   CAS 4478-93-7
别名: 萝卜硫素

Sulforaphane 是存在于多种蔬菜中的一种天然异硫氰酸酯。它可以激活Nrf2,并通过 AMPK 依赖性信号传导抑制高糖诱导的胰腺癌。它增加肿瘤抑制蛋白的转录并抑制组蛋白脱乙酰酶的活性,具有抗癌和抗炎活性。

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Sulforaphane Chemical Structure
Sulforaphane, CAS 4478-93-7
规格 价格/CNY 货期 数量
5 mg ¥ 297 现货
10 mg ¥ 492 现货
25 mg ¥ 1,120 现货
50 mg ¥ 1,920 现货
100 mg ¥ 3,730 现货
500 mg ¥ 7,880 现货
其他形式的 Sulforaphane:
产品目录号及名称: Sulforaphane (TQ0207)
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纯度: 99.79%
纯度: 98.29%
纯度: 95%
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天然产物信息
生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Sulforaphane is derived from the consumption of cruciferous vegetables, such as broccoli. It has shown anticancer and cardioprotective activities.
体外活性 Sulforaphane induces a cell cycle arrest in a dose-dependent manner, followed by cell death. This sulforaphane-induced cell cycle arrest was correlated with an increased expression of cyclins A and B1. Sulforaphane induces cell death via an apoptotic process. Sulforaphane inhibits the reinitiation of growth and diminishes cellular viability in quiescent colon carcinoma cells (HT29) and has lower toxicity on differentiated CaCo2 cells [1]. Pre-treatment of H9c2 rat myoblasts with sulforaphane decreases the apoptotic cell number and the expression of pro-apoptotic proteins, as well as the doxorubicin-induced increase in mitochondrial membrane potential. Furthermore, sulforaphane increases the mRNA and protein expression of heme oxygenase-1, which consequently reduces the levels of reactive oxygen species in the mitochondria which are induced by doxorubicin [2].
体内活性 Administration of sulforaphane reduces the incidence, multiplicity, and weights and delays the development of the mammary tumors evoked by a single dose of DMBA in female SD rats [3].
细胞实验 HT29 cells are seeded at low density (5×10^4 cells/mL) in 35- or 120-mm diameter Primaria dishes in standard medium containing 5% FCS. One day after seeding, the medium is changed, and HT29 cells are treated with sulforaphane (0-30 μM). An equivalent amount of the solvent (ethanol) is added to control cells (0.2% final concentration). The drug effect on cellular viability is evaluated using the MTT assay [1].
动物实验 At age 47, 48, 49, 50, and 51 days, each animal receives by gavage either 0.5 mL of Emulphor EL-620 alone or the specified doses (75, 100, or 150 μM daily) of sulforaphane or compound 2, 3, or 4 in 0.5 mL of Emulphor EL-620. On day 50, 3 hr after administration of the vehicle or protector, all rats also receive an intragastric instillation of 8.0 mg of DMBA dissolved in 1.0 mL of sesame oil. This dose of DMBA is selected to produce a substantial tumor incidence, but not one so high as to overwhelm a potential chemoprotective effect. The animals are examined once weekly for the appearance and location of palpable tumors. At age 202 days, i.e., 152 days after carcinogen administration, all animals are euthanized with ether and weighed. The tumors are separated from the fat and connective tissue by dissection weighed and fixed in buffered 10% formalin. All tumors are identified microscopically by examination of stained sections [3].
别名 萝卜硫素
分子量 177.29
分子式 C6H11NOS2
CAS No. 4478-93-7

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 60 mg/mL (338.43 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.6405 mL 28.2024 mL 56.4048 mL 141.0119 mL
5 mM 1.1281 mL 5.6405 mL 11.281 mL 28.2024 mL
10 mM 0.564 mL 2.8202 mL 5.6405 mL 14.1012 mL
20 mM 0.282 mL 1.4101 mL 2.8202 mL 7.0506 mL
50 mM 0.1128 mL 0.564 mL 1.1281 mL 2.8202 mL
100 mM 0.0564 mL 0.282 mL 0.564 mL 1.4101 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Gamet-Payrastre L, et al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res. 2000 Mar 1;60(5):1426-33. 2. Li B, et al. Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells. Int J Mol Med. 2015 Jul;36(1):53-64. 3. Zhang Y, et al. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornylisothiocyanates. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3147-50.
TBHQ ML385 (-)-Perillaldehyde toralactone Carlinoside Astilbin Ezetimibe Hesperin

相关化合物库

该产品包含在如下化合物库中:
抗癌临床化合物库 抗癌药物库 抗癌活性化合物库 临床期小分子药物库 抗癌天然产物库 药物功能重定位化合物库 天然产物库 抗胰腺癌化合物库 抗肥胖化合物库 表观遗传库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
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% ddH2O
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技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Sulforaphane 4478-93-7 Apoptosis Chromatin/Epigenetic DNA Damage/DNA Repair Immunology/Inflammation Nrf2 HDAC 萝卜硫素 Keap1-Nrf2 inhibit Inhibitor Histone deacetylases inhibitor

 

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