Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sulforaphane 是存在于多种蔬菜中的一种天然异硫氰酸酯。它可以激活Nrf2,并通过 AMPK 依赖性信号传导抑制高糖诱导的胰腺癌。它增加肿瘤抑制蛋白的转录并抑制组蛋白脱乙酰酶的活性,具有抗癌和抗炎活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 297 | 现货 | ||
10 mg | ¥ 492 | 现货 | ||
25 mg | ¥ 1,120 | 现货 | ||
50 mg | ¥ 1,920 | 现货 | ||
100 mg | ¥ 3,730 | 现货 | ||
500 mg | ¥ 7,880 | 现货 |
产品描述 | Sulforaphane is derived from the consumption of cruciferous vegetables, such as broccoli. It has shown anticancer and cardioprotective activities. |
体外活性 | Sulforaphane induces a cell cycle arrest in a dose-dependent manner, followed by cell death. This sulforaphane-induced cell cycle arrest was correlated with an increased expression of cyclins A and B1. Sulforaphane induces cell death via an apoptotic process. Sulforaphane inhibits the reinitiation of growth and diminishes cellular viability in quiescent colon carcinoma cells (HT29) and has lower toxicity on differentiated CaCo2 cells [1]. Pre-treatment of H9c2 rat myoblasts with sulforaphane decreases the apoptotic cell number and the expression of pro-apoptotic proteins, as well as the doxorubicin-induced increase in mitochondrial membrane potential. Furthermore, sulforaphane increases the mRNA and protein expression of heme oxygenase-1, which consequently reduces the levels of reactive oxygen species in the mitochondria which are induced by doxorubicin [2]. |
体内活性 | Administration of sulforaphane reduces the incidence, multiplicity, and weights and delays the development of the mammary tumors evoked by a single dose of DMBA in female SD rats [3]. |
细胞实验 | HT29 cells are seeded at low density (5×10^4 cells/mL) in 35- or 120-mm diameter Primaria dishes in standard medium containing 5% FCS. One day after seeding, the medium is changed, and HT29 cells are treated with sulforaphane (0-30 μM). An equivalent amount of the solvent (ethanol) is added to control cells (0.2% final concentration). The drug effect on cellular viability is evaluated using the MTT assay [1]. |
动物实验 | At age 47, 48, 49, 50, and 51 days, each animal receives by gavage either 0.5 mL of Emulphor EL-620 alone or the specified doses (75, 100, or 150 μM daily) of sulforaphane or compound 2, 3, or 4 in 0.5 mL of Emulphor EL-620. On day 50, 3 hr after administration of the vehicle or protector, all rats also receive an intragastric instillation of 8.0 mg of DMBA dissolved in 1.0 mL of sesame oil. This dose of DMBA is selected to produce a substantial tumor incidence, but not one so high as to overwhelm a potential chemoprotective effect. The animals are examined once weekly for the appearance and location of palpable tumors. At age 202 days, i.e., 152 days after carcinogen administration, all animals are euthanized with ether and weighed. The tumors are separated from the fat and connective tissue by dissection weighed and fixed in buffered 10% formalin. All tumors are identified microscopically by examination of stained sections [3]. |
别名 | 萝卜硫素 |
分子量 | 177.29 |
分子式 | C6H11NOS2 |
CAS No. | 4478-93-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (338.43 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 5.6405 mL | 28.2024 mL | 56.4048 mL | 141.0119 mL |
5 mM | 1.1281 mL | 5.6405 mL | 11.281 mL | 28.2024 mL | |
10 mM | 0.564 mL | 2.8202 mL | 5.6405 mL | 14.1012 mL | |
20 mM | 0.282 mL | 1.4101 mL | 2.8202 mL | 7.0506 mL | |
50 mM | 0.1128 mL | 0.564 mL | 1.1281 mL | 2.8202 mL | |
100 mM | 0.0564 mL | 0.282 mL | 0.564 mL | 1.4101 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Sulforaphane 4478-93-7 Apoptosis Chromatin/Epigenetic DNA Damage/DNA Repair Immunology/Inflammation Nrf2 HDAC 萝卜硫素 Keap1-Nrf2 inhibit Inhibitor Histone deacetylases inhibitor