Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sulfasalazine (Azulfidine) 是一种合成的水杨酸衍生物,对含有弹性蛋白的结缔组织具有亲和力,并配制成前药。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 333 | 现货 | ||
1 g | ¥ 418 | 现货 | ||
5 g | ¥ 671 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 418 | 现货 |
产品描述 | Sulfasalazine (Azulfidine) is a synthetic salicylic acid derivative with affinity for connective tissues containing elastin and formulated as a prodrug |
体外活性 | Sulfasalazine, like methotrexate, enhances adenosine release at an inflamed site and that adenosine diminishes inflammation via occupancy of A2 receptors on inflammatory cells. [1] Sulfasalazine treatment for 4 hours inhibits kappaB-dependent transcription with an IC50 value of approximately 0.625 mM. Sulfasalazine (2.5 mM) results in cell death of T-lymphocytes in a dose- and time-dependent manner. Sulfasalazine but not 5ASA or sulfapyridine, strongly inhibits NF-kappaB activation and potently induces apoptosis in T-lymphocytes. [2] Sulfasalazine is cleaved into sulfapyridine and 5-aminosalicylic acid (5-ASA; mesalamine) by colonic bacteria, and the latter, too, is reported to suppress NF-kappaB activity. Sulfasalazine but not its cleaved form 5-ASA causes a dose-dependent inhibition of glioma growth, this effect is entirely attributable to the inhibition of cystine uptake via the system x(c)(-) cystine-glutamate transporter. Sulfasalazine inhibits cystine uptake causing a chronic depletion of intracellular GSH and consequently compromised cellular redox defense which stymied tumor growth. [3] |
体内活性 | Sulfasalazine markedly decreases the number of leukocytes that accumulated in the inflamed (carrageenan, 2 mg/ml) air pouch in the murine air pouch model of inflammation. Sulfasalazine treatment promotes a marked increase in splenocyte 5-aminoimidazole-4-carboxamidoribonucleotide (AICAR) concentration, which is consistent with the in vitro observation that sulfasalazine inhibits AICAR transformylase. [1] |
细胞实验 | Sulfasalazine is dissolved in culture medium. SW620 cells are grown in Dulbecco's modified Eagle medium, supplemented with 10% heat-inactivated FCS, 2 mmol/liter glutamine, and 1% (wt/vol) penicillin/streptomycin. SW620 cells are transfected with the 3xIgkBLuc reporter construct. After 18 h, cells are incubated with either medium alone or with sulfasalazine (0.1, 0.2, 0.5, 1, 2, 5 mM) before stimulation with TNFα, LPS, or PMA. Luciferase assay is performed[1]. |
别名 | 柳氮磺吡啶, NSC 667219, Azulfidine |
分子量 | 398.39 |
分子式 | C18H14N4O5S |
CAS No. | 599-79-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 200 mg/mL (502.02 mM), Sonification/heating is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.5101 mL | 12.5505 mL | 25.101 mL | 62.7526 mL |
5 mM | 0.502 mL | 2.5101 mL | 5.0202 mL | 12.5505 mL | |
10 mM | 0.251 mL | 1.2551 mL | 2.5101 mL | 6.2753 mL | |
20 mM | 0.1255 mL | 0.6275 mL | 1.2551 mL | 3.1376 mL | |
50 mM | 0.0502 mL | 0.251 mL | 0.502 mL | 1.2551 mL | |
100 mM | 0.0251 mL | 0.1255 mL | 0.251 mL | 0.6275 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Sulfasalazine 599-79-1 Apoptosis Autophagy Immunology/Inflammation Microbiology/Virology Neuroscience NF-Κb Ferroptosis NF-κB COX Antibacterial Antibiotic Nuclear factor-κB 柳氮磺吡啶 Inhibitor NSC 667219 Nuclear factor-kappaB Bacterial inhibit NSC667219 NSC-667219 Azulfidine inhibitor