Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sodium butanoate (Sodium Butyrate) 是具有潜在抗肿瘤活性的butyrate 钠盐。Butyrate 是一种短链脂肪酸,与 I 类和 II 类组蛋白脱乙酰酶(HDAC) 的锌位点竞争性结合。
产品描述 | Sodium butanoate (Sodium Butyrate) is the sodium salt of butyrate with potential antineoplastic activity. Butyrate, a short chain fatty acid, competitively binds to the zinc sites of class I and II histone deacetylases (HDACs). |
体外活性 | Sodium butyrate能够增加组蛋白和特异性蛋白-1乙酰化,同时抑制3-硝基丙酸产生神经毒性.在亨廷顿氏病的R6 /2转基因小鼠模型中,Sodium butyrate能够延缓后遗症的发作和延长生存周期. |
体内活性 | 在结肠粘膜中,Sodium butyrate能够产生抗炎和抗癌作用,降低氧化应激,从而抑制结肠癌细胞的生长。在RG / C2和AA / Cl腺瘤细胞系中,Sodium butyrate能够诱导细胞产生凋亡。 |
细胞实验 | Cells are seeded at a density of 2,000 cells/well in 96-well plates with 200 μL culture medium containing Sodium Butyrate at different concentrations. Then, the cells are consecutively cultured for 72 h. Every 24 h, 20 μL 5 mg/mL MTT solution is added into the corresponding well, and the cells are cultured for another 4 h. Then, the solution is replaced with 150 μL dimethylsulfoxide, followed by gentle agitation of the plates for 15 min at room temperature. Finally, the absorbance at 492 nm is measured to represent the cell viability. |
别名 | Sodium Butyrate, 丁酸钠, Butanoic acid sodium salt |
分子量 | 110.09 |
分子式 | C4H7NaO2 |
CAS No. | 156-54-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 1.1 mg/mL (10 mM), Sonication is recommended.
H2O: 100 mg/mL (908.35 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 9.0835 mL | 45.4174 mL | 90.8348 mL | 227.0869 mL |
5 mM | 1.8167 mL | 9.0835 mL | 18.167 mL | 45.4174 mL | |
10 mM | 0.9083 mL | 4.5417 mL | 9.0835 mL | 22.7087 mL | |
H2O | 20 mM | 0.4542 mL | 2.2709 mL | 4.5417 mL | 11.3543 mL |
50 mM | 0.1817 mL | 0.9083 mL | 1.8167 mL | 4.5417 mL | |
100 mM | 0.0908 mL | 0.4542 mL | 0.9083 mL | 2.2709 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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