Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Scutellarin (Scutellarein-7-glucuronide) 是从黄芩中分离的黄酮,可抑制破骨细胞中 RANKL 介导的 MAPK 和 NF-κB 信号通路,并下调 HCC 细胞中的 STAT3/Girdin/Akt 信号通路。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 357 | 现货 | ||
25 mg | ¥ 642 | 现货 | ||
50 mg | ¥ 1,177 | 现货 | ||
100 mg | ¥ 1,766 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 374 | 现货 |
产品描述 | Scutellarin (Scutellarein-7-glucuronide), an active flavone isolated from Scutellaria baicalensis, can inhibit RANKL-mediated MAPK and NF-κB signaling pathway in osteoclasts, and down-regulate the STAT3/Girdin/Akt signaling in HCC cells. |
体外活性 | Scutellarin treatment significantly reduces HepG2 cell viability in a dose-dependent manner. It also inhibits migration and invasion of HCC cells in vitro. Scutellarin treatment significantly reduces STAT3 and Girders of actin filaments (Girdin) expression, STAT3 and Akt phosphorylation in HCC cells. The introduction of STAT3 overexpression restores the scutellarin-downregulated Girdin expression, Akt activation, migration and invasion of HCC cells. Moreover, induction of Girdin overexpression completely abrogates the inhibition of scutellarin on the Akt phosphorylation, migration and invasion of HCC cells. Scutellarin can inhibit HCC cell metastasis in vivo, and migration and invasion in vitro by down-regulating the STAT3/Girdin/Akt signaling[1]. Scutellarin selectively enhances Akt phosphorylation[2]. Scutellarin is a putative therapeutic agent as it has been found to not only suppress microglial activation thus ameliorating neuroinflammation, but also enhance astrocytic reaction. Acutellarin amplifies the astrocytic reaction by upregulating the expression of neurotrophic factors among others thus indicating its neuroprotective role. Remarkably, the effects of scutellarin on reactive astrocytes are mediated by activated microglia supporting a functional 'cross-talk' between the two glial types[3]. Scutellarin can suppress RANKL-mediated osteoclastogenesis, the function of osteoclast bone resorption, and the expression levels of osteoclast-specific genes (tartrate-resistant acid phosphatase (TRAP), cathepsin K, c-Fos, NFATc1). Further investigation indicates that Scutellarin can inhibit RANKL-mediated MAPK and NF-κB signaling pathway, including JNK1/2, p38, ERK1/2, and IκBα phosphorylation[5]. |
体内活性 | Scutellarin (50 mg/kg/day) significantly mitigates the lung and intrahepatic metastasis of HCC tumors in vivo. The numbers of lung and intrahepatic metastatic tumors in the scutellarin-treated group are significantly less compared to the controls[1]. The rats treated with Scutellarin display a significant alleviation in neurobehavioral deficits compared to the SAH group. Scutellarin enhanced eNOS expression compared with SAH rats[4]. |
细胞实验 | HepG2 cells (1×105/well) are cultured in 96-well plates and treated in triplicate with scutellarin at concentrations of 5, 10, 20, 30, and 100 μM or vehicle alone for 24 h. The cellular viability is tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and is expressed as a percentage of proliferation versus controls. |
别名 | 黄岑素, 野黄芩苷, Breviscapin, Breviscapinun, Breviscapine, Scutellarein-7-glucuronide |
分子量 | 462.36 |
分子式 | C21H18O12 |
CAS No. | 27740-01-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50mg/mL(108.14 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.1628 mL | 10.8141 mL | 21.6282 mL | 54.0704 mL |
5 mM | 0.4326 mL | 2.1628 mL | 4.3256 mL | 10.8141 mL | |
10 mM | 0.2163 mL | 1.0814 mL | 2.1628 mL | 5.407 mL | |
20 mM | 0.1081 mL | 0.5407 mL | 1.0814 mL | 2.7035 mL | |
50 mM | 0.0433 mL | 0.2163 mL | 0.4326 mL | 1.0814 mL | |
100 mM | 0.0216 mL | 0.1081 mL | 0.2163 mL | 0.5407 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
白苏 | Perilla frutescens (L.) Britt. | 温 | 辛 | 肺, 大肠 |
半枝莲 | Scutellaria barbata D. Don. | 寒 | 辛, 苦 | 肺, 肝, 肾 |
臭茉莉 | C.philippinum Schauer var.simplex Mold-enke | 平 | 苦 | 心, 脾, 肾 |
大车前 | Plantago major L. | 寒 | 甘 | 肝, 肾, 膀胱 |
灯盏花 | Erigeron brevisca pus (Vant.)Hand.-Mazz. | 温 | 辛, 微苦 | 心, 肝 |
灯盏细辛 | Erigeron brevisca pus (Vant.)Hand.-Mazz. | 温 | 辛, 微苦 | 心, 肝 |
中成药名称 | 处方组成 | 主治疾病 | 中成药类型 |
丹参益心胶囊 | 三七,回心草,灯盏细辛,紫丹参,制何首乌,延胡索 | 口服,一次3-4粒,一日三次。 | 祛瘀药 |
参鹿扶正胶囊 | 人参,熟地黄,杜仲,枸杞子,巴戟天,牛膝,茯苓,沙棘,胡芦巴,天冬,五味子,五加皮,肉苁蓉,鹿角胶,山药,酸枣仁,桑椹,白术,半枝莲,猫爪草 | 口服,一次2~4粒,一日3次。 | 扶正药 |
丹灯通脑软胶囊 | 丹参,灯盏细辛,川芎,葛根 | 口服,一次 4 粒,一日 3 次。 30 天为 1 个疗程。 | 祛瘀药 |
安康欣胶囊 | 半枝莲,山豆根,蒲公英,鱼腥草,夏枯草,石上柏,枸杞子,穿破石,人参,黄芪,鸡血藤,灵芝,黄精,白术,党参,淫羊藿,兔丝子,丹参 | 每日三次,每次4-6粒,饭后温开水送服。 | 安神药 |
灯盏花滴丸 | 灯盏细辛 | 口服。一次14丸,一日3次。 | 祛瘀药 |
丹鳖胶囊 | 丹参,三七,三棱,莪术,桃仁(去皮),当归,鳖甲,海藻,杜仲(盐炒),白术(炒),半枝莲,桂枝 | 口服。一次5粒,一日3次。 | 祛瘀药 |
灯盏细辛软胶囊 | 灯盏细辛 | 口服,一次2 ~3粒,一日3次,或遵医嘱。 | 祛瘀药 |
灯盏细辛胶囊 | 灯盏细辛 | 口服。一次2~3粒,一日3次,或遵医嘱。 | 祛瘀药 |
灯盏细辛颗粒 | 灯盏细辛 | 口服。一 次 1 ? 2 袋 9一日 次 。 | 祛瘀药 |
半枝莲片 | 半枝莲 | 口服,一次5片,一日3次。 | 清热药 |
灯银脑通胶囊 | 灯盏细辛,银杏叶,三七,满山香 | 口服,一次2粒,一日3次,14天为一疗程;或遵医嘱。 | 祛瘀药 |
白沙糖浆 | 白杨,沙糖木,半枝莲,苦参,橘子 | 口服,一次15毫升,一日3次。 | 止咳、化痰、平喘药 |
丹灯通脑片 | 丹参,灯盏细辛,川芎,葛根 | 口服。一次4片,一日3次。一个月为1个疗程。 | 祛瘀药 |
参鹿扶正片 | 人参,熟地黄,杜仲,枸杞子,巴戟天,牛膝,茯苓,沙棘,胡芦巴,天冬,五味子,五加皮,肉苁蓉,鹿角胶,山药,酸枣仁,桑椹,白术,半枝莲,猫爪草 | 口服,一次2~4粒,一日3次;或遵医嘱。 | 扶正药 |
丹灯通脑滴丸 | 丹参,灯盏细辛,川芎,葛根 | 口服,一次 4 粒,一日 3 次。 30 天为 1 个疗程。 | 祛瘀药 |
复方肾炎片 | 丹参,黄芪,黄芩,茯苓,牵牛子,车前子,白茅根,芦根,黄精,半枝莲,蒲黄,益母草,菟丝子,茜草,山楂 | 口服,一次3片,一日3次。 | 祛湿药 |
半枝莲胶囊 | 半枝莲 | 口服。一次5粒,一日3次。 | 清热药 |
丹灯通脑胶囊 | 丹参,灯盏细辛,川芎,葛根 | 口服,一次 4 粒,一日 3 次。 30 天为 1 个疗程。 | 祛瘀药 |
灯盏生脉胶囊 | 灯盏细辛,人参,五味子,麦冬 | 口服。一次2粒,一日3次。饭后30分钟服用。两个月为一疗程,疗程可连续。巩固疗效或预防复发,一次1粒,一日3次。 | 祛瘀药 |
参莲胶囊 | 苦参,山豆根,半枝莲,防己,三棱,莪术,丹参,补骨脂,苦杏仁,乌梅,白扁豆 | 口服,每次6粒,一日3次。 | 扶正药 |
方剂名称 | 处方组成 | 剂型 | 处方来源 |
泽下汤 | 人参,当归,白芍,生地黄,白苏,火麻仁,石斛,山药,野料豆,大枣, | 汤剂 | 《医醇剩义》卷二。 |
百花膏3 | 透骨草,忍冬藤,蒲公英,鹤虱,九龙藤,天麻,墨旱莲,半枝莲,地杨梅,豨莶草,苍耳草,紫花地丁,地锦草,鱼蓼,大小青,薄荷,灵芝,鱼腥草,血见愁,淡竹叶,南天竹,枸杞,头橘树头,枳叶,五加叶,接骨木,石楠,蜈蚣,萹蓄,马齿苋,石荠苎,蛇床叶,长青草,慎火草,太湖葱 | 膏剂 | 《解围元薮》卷四。 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Scutellarin 27740-01-8 Cytoskeletal Signaling JAK/STAT signaling Microbiology/Virology PI3K/Akt/mTOR signaling Proteases/Proteasome Stem Cells Akt HIV Protease STAT 黄岑素 PKB Inhibitor 野黄芩苷 inhibit HIV Protein kinase B Human immunodeficiency virus Breviscapin Breviscapinun Breviscapine Scutellarein-7-glucuronide inhibitor