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SYBR GREEN II

SYBR GREEN II

产品编号 TD0005   CAS 172827-25-7
别名: SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade), SUPER Green II (效果同SYBR Green II)RNA染料(10,000× DMSO溶液)(电泳级)

1) Non-toxicity: belong to flower-stem dye, easy to biodegrade, no carcinogenic toxicity. 2) High sensitivity: at least 100pg ssDNA or RNA can be detected, which is 25 ~ 100 times higher than EB staining. 3) High SIGNal-to-noise ratio: the sample fluorescence signal is strong, while the background signal is low. 4) Simple operation: like EB, the dye does not degrade in the prefabricated gel and electrophoresis process;The dyeing process after electrophoresis takes only 30 minutes without decoloring or washing, and can be observed directly with ultraviolet gel transmission or visible light transmission.

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SYBR GREEN II
SYBR GREEN II, CAS 172827-25-7
规格 价格/CNY 货期 数量
100 μL * 10 mM (in DMSO) ¥ 1,687 待询
千万补贴 助力科研
BCA蛋白浓度测定试剂盒限时半价
Venetoclax限时半价
产品目录号及名称: SYBR GREEN II (TD0005)
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存储 & 溶解度
产品描述 1) Non-toxicity: belong to flower-stem dye, easy to biodegrade, no carcinogenic toxicity. 2) High sensitivity: at least 100pg ssDNA or RNA can be detected, which is 25 ~ 100 times higher than EB staining. 3) High SIGNal-to-noise ratio: the sample fluorescence signal is strong, while the background signal is low. 4) Simple operation: like EB, the dye does not degrade in the prefabricated gel and electrophoresis process;The dyeing process after electrophoresis takes only 30 minutes without decoloring or washing, and can be observed directly with ultraviolet gel transmission or visible light transmission.
体外活性 Staining RNA Following Electrophoresis Perform electrophoresis on nondenaturing gels or on denaturing urea/polyacrylamide or formaldehyde/agarose gels according to standard techniques. For nondenaturing gels and denaturing urea/polyacrylamide gels, a 1:10,000 dilution in TBE (pH =8) is recommed. For denaturing formaldehyde/agarose gels, a 1:5000 dilution in TBE is recommed. For optimal sensitivity, the pH of the staining solution used for staining is between 7.5 and 8.0 (preferably pH 8.0).Place the gel in a staining container, and add enough staining solution to cover the gel. Protect the staining container from light . Shake the gel gently at room temperature. The staining time is typically 10–40 minutes for polyacrylamide gels and 20–40 minutes for agarose gels. Illuminate the stained gel using 300 nm ultraviolet transillumination, or for greater sensitivity, 254 nm epi-illumination. Photograph the gel with Polaroid 667 black-and-white print film using a SYBR Green gel stain photographic filter.
别名 SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade), SUPER Green II (效果同SYBR Green II)RNA染料(10,000× DMSO溶液)(电泳级)
分子量 N/A
CAS No. 172827-25-7

存储

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

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SYBR GREEN II 172827-25-7 SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade) Electrophoresis Grade SUPER Green II (效果同SYBR Green II)RNA染料(10,000× DMSO溶液)(电泳级) Inhibitor inhibitor inhibit

 

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