keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
1) Non-toxicity: belong to flower-stem dye, easy to biodegrade, no carcinogenic toxicity. 2) High sensitivity: at least 100pg ssDNA or RNA can be detected, which is 25 ~ 100 times higher than EB staining. 3) High SIGNal-to-noise ratio: the sample fluorescence signal is strong, while the background signal is low. 4) Simple operation: like EB, the dye does not degrade in the prefabricated gel and electrophoresis process;The dyeing process after electrophoresis takes only 30 minutes without decoloring or washing, and can be observed directly with ultraviolet gel transmission or visible light transmission.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μL * 10 mM (in DMSO) | ¥ 1,687 | 待询 |
产品描述 | 1) Non-toxicity: belong to flower-stem dye, easy to biodegrade, no carcinogenic toxicity. 2) High sensitivity: at least 100pg ssDNA or RNA can be detected, which is 25 ~ 100 times higher than EB staining. 3) High SIGNal-to-noise ratio: the sample fluorescence signal is strong, while the background signal is low. 4) Simple operation: like EB, the dye does not degrade in the prefabricated gel and electrophoresis process;The dyeing process after electrophoresis takes only 30 minutes without decoloring or washing, and can be observed directly with ultraviolet gel transmission or visible light transmission. |
体外活性 | Staining RNA Following Electrophoresis Perform electrophoresis on nondenaturing gels or on denaturing urea/polyacrylamide or formaldehyde/agarose gels according to standard techniques. For nondenaturing gels and denaturing urea/polyacrylamide gels, a 1:10,000 dilution in TBE (pH =8) is recommed. For denaturing formaldehyde/agarose gels, a 1:5000 dilution in TBE is recommed. For optimal sensitivity, the pH of the staining solution used for staining is between 7.5 and 8.0 (preferably pH 8.0).Place the gel in a staining container, and add enough staining solution to cover the gel. Protect the staining container from light . Shake the gel gently at room temperature. The staining time is typically 10–40 minutes for polyacrylamide gels and 20–40 minutes for agarose gels. Illuminate the stained gel using 300 nm ultraviolet transillumination, or for greater sensitivity, 254 nm epi-illumination. Photograph the gel with Polaroid 667 black-and-white print film using a SYBR Green gel stain photographic filter. |
别名 | SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade), SUPER Green II (效果同SYBR Green II)RNA染料(10,000× DMSO溶液)(电泳级) |
分子量 | N/A |
CAS No. | 172827-25-7 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SYBR GREEN II 172827-25-7 SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade) Electrophoresis Grade SUPER Green II (效果同SYBR Green II)RNA染料(10,000× DMSO溶液)(电泳级) Inhibitor inhibitor inhibit