keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SYBR GREEN 1 是一种不对称花青染料,用于检测核酸。它由一个 N-烷基化的苯并噻唑或苯并恶唑环系统组成,通过一甲基桥连接到吡啶鎓或喹啉鎓环系统。 SYBR Green I 与 dsDNA 的小沟结合,在 480 nm 波长处被激发。它具有 520 nm 的峰值荧光发射。
产品描述 | SYBR Green I is an asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm. |
体外活性 | Precasting SYBR Green I Gels:Precast nondenaturing polyacrylamide or agarose gels with SYBR Green I staining solution, which is diluted from the SYBR Green I stock reagent 1:10,000 prior to pouring the gel. The DNA detection limit for gels precast with SYBR Green I stain may be slightly higher (on the order of 30 to 40 pg/band) than for gels stained after electrophoresis (less than 20 pg/band), and the migration rate of DNA fragments in SYBR Green I gels may be significantly slower than the migration rate of the same fragments in a gel containing no dye. |
别名 | SUPER Green I (效果同SYBR Green I)核酸染料(10,000× DMSO溶液)(电泳级), SYBR(R) GREEN I NUCLEIC ACID GEL STAIN *10,000× concentrate in DMSO, SYBRGREENⅠ核苷酸胶体染料 |
分子量 | 509.74 |
分子式 | C32H37N4S |
CAS No. | 163795-75-3 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SYBR GREEN 1 163795-75-3 Others SUPER Green I (效果同SYBR Green I)核酸染料(10,000× DMSO溶液)(电泳级) SYBR(R) GREEN I NUCLEIC ACID GEL STAIN *10,000× concentrate in DMSO SYBRGREENⅠ核苷酸胶体染料 Inhibitor inhibitor inhibit