Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SMER28 是一种自噬小分子增强剂,通过不依赖于 mTOR 的机制诱导哺乳动物细胞中独立于雷帕霉素的自噬,并阻止淀粉样蛋白 β 肽的积累。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 192 | 现货 | ||
5 mg | ¥ 413 | 现货 | ||
10 mg | ¥ 578 | 现货 | ||
25 mg | ¥ 1,160 | 现货 | ||
50 mg | ¥ 2,230 | 现货 | ||
100 mg | ¥ 3,390 | 现货 | ||
200 mg | ¥ 4,920 | 现货 | ||
500 mg | ¥ 7,730 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 455 | 现货 |
产品描述 | SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells via an mTOR-independent mechanism and prevented the accumulation of amyloid beta peptide. |
体外活性 | SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment[1]. |
细胞实验 | MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10 μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies.(Only for Reference) |
分子量 | 264.12 |
分子式 | C11H10BrN3 |
CAS No. | 307538-42-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 26.4 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.7862 mL | 18.9308 mL | 37.8616 mL | 94.6539 mL |
5 mM | 0.7572 mL | 3.7862 mL | 7.5723 mL | 18.9308 mL | |
10 mM | 0.3786 mL | 1.8931 mL | 3.7862 mL | 9.4654 mL | |
20 mM | 0.1893 mL | 0.9465 mL | 1.8931 mL | 4.7327 mL | |
50 mM | 0.0757 mL | 0.3786 mL | 0.7572 mL | 1.8931 mL | |
100 mM | 0.0379 mL | 0.1893 mL | 0.3786 mL | 0.9465 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SMER28 307538-42-7 Autophagy inhibit Inhibitor SMER-28 SMER 28 inhibitor