SC79

产品编号: T2274 别名:

2-氨基-6-氯-alpha-氰基-3-(乙氧羰基)-4H-1-苯并吡喃-4-乙酸乙酯,SC 79

SC 79 是一个特异的、能透过血脑屏障的Akt激动剂，能够特异性结合Akt 的PH 结构域，激活胞质中Akt，并抑制 Akt 膜转位。

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CAS：305834-79-1

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生物活性化学信息储存和溶解度信息参考文献引用文献

产品描述

SC79, a selective Akt activator, may be used to enhance Akt activity in various pathological and physiological conditions.

体外活性

SC79 suppresses PHAKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells. SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. [1] SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells. [2]

体内活性

In the permanent focal cerebral ischemia mouse model, SC79 (0.04 mg/g, i.p.) inhibits the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival. [1]

激酶实验

Cytosolic phosphorylation of Akt: Hela cells are serum starved for 1 hr and treated with IGF (100ng/mL) or SC79 (4 μg/mL) for 30 minutes. Cells are lysed in Lysis buffer containing 250 mM Sucrose, 20 mM HEPES, 10 mM KCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM EGTA supplemented with protease inhibitors. Cells are passed through 25 g needle several times and kept on ice for 20 minutes. Total cell lysate is taken at this point. Cell lysates are centrifuged at 100,000 g for 30 minutes. Supernatant is collected as the cytosolic fraction. Pellet is washed with lysis buffer and represents the membrane fraction. Total cell lysate, cytosolic and membrane fractions are resolved by SDS-PAGE and analyzed for phospho-Akt (S473), Total Akt, Tubulin (cytosolic marker) and Orai1 (membrane marker) by western blotting.

细胞实验

HsSultan or NB4 cells (2.5 × 105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 μg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.(Only for Reference)

Cas No.

305834-79-1

分子式

C17H17ClN2O5

分子量

364.78

别名

2-氨基-6-氯-alpha-氰基-3-(乙氧羰基)-4H-1-苯并吡喃-4-乙酸乙酯;SC 79;SC79

参考文献

[1]Shi W, Tang Y, Zhi Y, et al. Akt inhibition-dependent downregulation of the Wnt/β-Catenin Signaling pathway contributes to antimony-induced neurotoxicity. Science of the Total Environment. 2020, 737: 140252.[2]Jo H, et al. Proc Natl Acad Sci U S A. 2012, 109(26), 10581-10586.[3]So EY, et al. BMC Cancer. 2014, 14, 548.[4]Wang X, Zhu P, Xu S, et al. Antimony,a novel nerve poison, triggers neuronal autophagic death via reactive oxygen species-mediated inhibition of the protein kinase B/mammalian target of rapamycin pathway[J]. The International Journal of Biochemistry & Cell Biology. 2019: 105561.

引用文献

[1]Chen H, He A, Li H, et al. TSSK4 upregulation in alveolar epithelial type-II cells facilitates pulmonary fibrosis through HSP90-AKT signaling restriction and AT-II apoptosis. Cell Death & Disease. 2021, 12(10): 1-1 [2]Liu Y, Lv H, Li X, et al. Cyclovirobuxine inhibits the progression of clear cell renal cell carcinoma by suppressing the IGFBP3-AKT/STAT3/MAPK-Snail signalling pathway. International Journal of Biological Sciences. 2021, 17(13): 3522-3537.[3]Zhang H, Xia P, Liu J, et al. ATIC inhibits autophagy in hepatocellular cancer through the AKT/FOXO3 pathway and serves as a prognostic signature for modeling patient survival. International Journal of Biological Sciences. 2021, 17(15): 4442-4458.[4]Xu Y, Jiang E, Shao Z, et al. LncRNA FENDRR in Carcinoma-Associated Fibroblasts Regulates the Angiogenesis of Oral Squamous Cell Carcinoma Through the PI3K/AKT Pathway. Frontiers in Oncology. 2021, 11.[5]Wang X, Zhu P, Xu S, et al. Antimony, a novel nerve poison, triggers neuronal autophagic death via reactive oxygen species-mediated inhibition of the protein kinase B/mammalian target of rapamycin pathway. The International Journal of Biochemistry & Cell Biology. 2019, 114: 105561 [6]Shi W, Tang Y, Zhi Y, et al Akt inhibition-dependent downregulation of the Wnt/β-Catenin Signaling pathway contributes to antimony-induced neurotoxicity. Science of the Total Environment. 2020, 737: 140252 [7]Chen C, Zhang H, Hou S, et al. Shenxian-Shengmai Oral Liquid Evoke Autophagy of Fibroblast to Attenuate Sinoatrial Node Fibrosis in Sick Sinus Syndrome Mice via the AKT/mTOR Pathway. Evidence-Based Complementary and Alternative Medicine. 2022

储存和溶解度

DMSO:36.5 mg/mL (100 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years

剂量换算 SC79 305834-79-1

对于各种应用，安全且有效的用药剂量是很有必要的了解更多

体内实验配液计算器

第一步：请输入动物实验的基本信息

剂量 mg/kg

每只动物体重 g

给药体积 ul

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO +

% +

% Tween 80 +

% ddH₂O

% DMSO +

重置计算

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

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已知质量的化合物在一定体积内形成的溶液的浓度

质量

浓度

体积

分子量

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制备已知体积和浓度溶液所需的化合物质量
将已知质量的化合物溶解到所需浓度所需的溶液体积
已知质量的化合物在一定体积内形成的溶液的浓度

浓度（起始）

体积（起始）

浓度（终点）

体积（终点）

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配液浓度

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总分子量

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技术支持

在抑制剂处理说明中可以找到您可能遇到的问题的答案。主题包括如何准备储备溶液，如何存储产品以及基于细胞的测定和动物实验需要特别注意的问题。

Keywords

SC79 inhibit 2-氨基-6-氯-alpha-氰基-3-(乙氧羰基)-4H-1-苯并吡喃-4-乙酸乙酯 Akt Protein kinase B Inhibitor SC-79 PKB SC 79 305834-79-1

SC79 相关推荐

相关化合物库推荐该产品包含在如下化合物库中：

注册成功

SC79

2-氨基-6-氯-alpha-氰基-3-(乙氧羰基)-4H-1-苯并吡喃-4-乙酸乙酯,SC 79

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