首页 工具
登录
购物车
SBI-0640756

SBI-0640756

产品编号 T5173   CAS 1821280-29-8
别名: SBI 0640756, SBI-756

SBI-0640756 (SBI-756) 是一种靶向 eIF4G1 并破坏 eIF4F 复合物的抑制剂。

TargetMol的所有产品和服务仅用于科学研究,不能被用于人体,我们也不向个人提供产品和服务。
SBI-0640756 Chemical Structure
SBI-0640756, CAS 1821280-29-8
规格 价格/CNY 货期 数量
1 mg ¥ 398 现货
2 mg ¥ 578 现货
5 mg ¥ 1,110 现货
10 mg ¥ 1,780 现货
25 mg ¥ 3,330 现货
50 mg ¥ 4,760 现货
100 mg ¥ 6,630 现货
1 mL * 10 mM (in DMSO) ¥ 1,210 现货
产品目录号及名称: SBI-0640756 (T5173)
点击图片重新获取验证码
选择批次  
纯度: 99.83%
更多批次查询请联系客服
生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 SBI-0640756 (SBI-756) (SBI-756) is a first-in-class inhibitor that targets eIF4G1 and disrupts the eIF4F complex.
体外活性 SBI-756 effectively dissociated eIF4G1 from the eIF4E in a dose-dependent manner, which was accompanied by a concomitant increase in 4E-BP1:eIF4E binding, reflective of impaired eIF4F complex formation. SBI-756 also inhibits the AKT/mTORC1 signaling and mTORC1 inhibition disrupts the eIF4F complex via activation of 4E-BPs. Whereas torin1 induced dissociation of eIF4G1 from eIF4E in WT but not in 4E-BP DKO MEFs, SBI-756 reduced eIF4G1:eIF4E association in both WT and 4E-BP DKO MEFs. Likewise, SBI-756 but not torin1 attenuated the proliferation of E1A/RAS-transformed 4E-BP DKO MEFs.
体内活性 Administration of SBI-756 only, starting 11 weeks after genetic inactivation of Ink4a and induction of NRasQ61E (about 10–14 days prior to tumor appearance), delayed tumor onset (from 20–26 weeks) and reduced tumor incidence, by 50%, compared with the control non-treated group. The growth of established tumors was largely inhibited by treatment with either BRAFi alone or a combination of BRAFi plus SBI-756.
细胞实验 Cells were rinsed with PBS and lysed as previously described. Protein concentration was determined using Coomassie Plus Protein Assay Reagent. Equal amounts of cell lysate proteins (50 μg) were separated on SDS-PAGE and transferred to polyvinylidene difluoride membranes. Membranes were blocked (5% BSA/TBST, 1 h) and incubated with primary antibodies (1 h at room temperate or overnight at 4°C), with shaking. Following three TBST washes, membranes were incubated for 1 h at room temperature secondary antibodies (1:10,000). Detection and quantifications were made using Odyssey Infrared Imaging System, or by exposing them to X-ray film. Antibodies against p-AKT, p-PRAS40, p-IKK, p-IκB, p-TSC, p-mTOR, p-p70S6K, p-RPS6, p-4E-BP1, pSGK3, AKT, PRAS40, IKK, IκB, mTOR, p70S6K, RPS6, 4E-BP1, GSK3, eIF4G1, and eIF4E were purchased from Cell Signaling Technology. Antibodies against β-actin and α-tubulin were obtained from Santa Cruz Biotechnology. Secondary antibodies were goat anti-rabbit Alexa-680 F(ab')2 and goat anti-mouse IRDye 800 F(ab')2. All antibodies were used according to the suppliers' recommendations.
别名 SBI 0640756, SBI-756
分子量 404.82
分子式 C23H14ClFN2O2
CAS No. 1821280-29-8

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: Insoluble

DMSO: 50 mg/mL (123.51 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4702 mL 12.3512 mL 24.7023 mL 61.7558 mL
5 mM 0.494 mL 2.4702 mL 4.9405 mL 12.3512 mL
10 mM 0.247 mL 1.2351 mL 2.4702 mL 6.1756 mL
20 mM 0.1235 mL 0.6176 mL 1.2351 mL 3.0878 mL
50 mM 0.0494 mL 0.247 mL 0.494 mL 1.2351 mL
100 mM 0.0247 mL 0.1235 mL 0.247 mL 0.6176 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
=
X
X
X
=
X
=
/
g/mol

输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Feng Y, et al. SBI-0640756 Attenuates the Growth of Clinically Unresponsive Melanomas by Disrupting the eIF4F Translation Initiation Complex. Cancer Res. 2015 Dec 15;75(24):5211-8.
Necrostatin-1 Vemurafenib (E)-Daporinad Hemin Dantrolene sodium hemiheptahydrate mTOR inhibitor-1 (+)-JQ-1 Rabusertib

相关化合物库

该产品包含在如下化合物库中:
激酶抑制剂库 已知活性化合物库 内质网应激化合物库 抑制剂库 细胞凋亡化合物库 自噬库 经典已知活性库 抗衰老化合物库 细胞重编程化合物库 NO PAINS 化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

SBI-0640756 1821280-29-8 Apoptosis Autophagy PERK Inhibitor SBI 0640756 SBI 756 SBI756 Eukaryotic Initiation Factor (eIF) inhibit SBI0640756 SBI-756 inhibitor

 

陶术
生物
TargetMol®中国区唯一合作伙伴
点击进入陶术生物官网陶术生物
联系我们
400-820-0310

上海市静安区江场三路238号8楼