Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Rostafuroxin (PST 2238) 是洋地黄毒苷衍生物,是一种口服有效的 Na+,K+-ATPase 抑制剂,具有抗高血压和抗 RSV 活性,用于研究治疗原发性高血压的试验。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 247 | 现货 | ||
2 mg | ¥ 359 | 现货 | ||
5 mg | ¥ 616 | 现货 | ||
10 mg | ¥ 1,120 | 现货 | ||
25 mg | ¥ 2,120 | 现货 | ||
50 mg | ¥ 3,730 | 现货 | ||
100 mg | ¥ 5,360 | 待询 | ||
1 mL * 10 mM (in DMSO) | ¥ 688 | 现货 |
产品描述 | Rostafuroxin (PST 2238) has been used in trials studying the treatment of Essential Hypertension. |
靶点活性 | K+-ATPase:1.5 nM, Na+-ATPase:1.5 nM |
激酶实验 | Kinase assay: Activity assays are conducted using Lance Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) technology from Perkin-Elmer. Briefly, 10 ng/mL FLT3 enzyme, a serial diluted G-749, 80 nM substrate of ULight-poly-GT peptide and variable amounts of ATP (8.5 μM to 1088 μM) are mixed in kinase assay buffer (50 mM HEPES pH 7.5, 10 mM MgCl2, 1 mM EGTA, 2 mM DTT and 0.01% Tween-20) and are added to a 384-well OptiPlate-384 in a volume of 10 μL. Kinase reactions are incubated at room temperature for up to 1 h and then stopped by the addition of 5 μL of 10 mM EDTA. A volume of 5 μL of the specific Eu-labeled-anti-phosphopeptide antibody diluted in LANCE Detection Buffer is then added to a final concentration of 2 nM. After 30-minute incubation, assay plates are incubated at 23°C and the LANCE signal is measured on an EnVision Multilabel Reader. Excitation wavelength is set at 320 nm and emission monitored at 615 nm (donor) and 665 nm (acceptor). The IC50 is calculated using nonlinear regression analysis analysis by GradPad Prism 5. |
别名 | PST 2238 |
分子量 | 374.51 |
分子式 | C23H34O4 |
CAS No. | 156722-18-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (133.5 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.6702 mL | 13.3508 mL | 26.7016 mL | 66.7539 mL |
5 mM | 0.534 mL | 2.6702 mL | 5.3403 mL | 13.3508 mL | |
10 mM | 0.267 mL | 1.3351 mL | 2.6702 mL | 6.6754 mL | |
20 mM | 0.1335 mL | 0.6675 mL | 1.3351 mL | 3.3377 mL | |
50 mM | 0.0534 mL | 0.267 mL | 0.534 mL | 1.3351 mL | |
100 mM | 0.0267 mL | 0.1335 mL | 0.267 mL | 0.6675 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Rostafuroxin 156722-18-8 Membrane transporter/Ion channel Microbiology/Virology ATPase RSV ATP1A1 Respiratory syncytial virus Na+/K+ ATPase phosphorylation inhibit PST-2238 Tyr845 Sodium potassium pump EGFR PST2238 antihypertensive RSV-GFP PST 2238 Inhibitor digitoxigenin Ouabain inhibitor