首页 NF-Κb IκB/IKK Resveratrol
Resveratrol
编号 T1558     别名: trans-Resveratrol, SRT 501
CAS 501-36-0     分子式 C14H12O3     分子量 228.24
靶点: COX-1; COX-2; DNA polymerase α; IKKβ; LOX; Quinone reductase 2; SIRT1; SIRT2; COX1; DNA polymerases α; DNA polymerases δ;
Resveratrol is a polyphenolic phytoalexin with antioxidant and chemopreventive activities. It has a wide spectrum of targets including COX, SIRT, LOC, etc.
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规格 库存 单价 数量
200 mg 上海现货 442.00
500 mg 上海现货 964.00
1 mL * 10 mM (in DMSO) 上海现货 148.00
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生物活性

产品描述

Resveratrol is a polyphenolic phytoalexin with antioxidant and chemopreventive activities. It has a wide spectrum of targets including COX, SIRT, LOC, etc.

靶点活性

COX-1,IC50:1.1μM

COX-2,1.3 μM (cell free)

DNA polymerase α,3.3μM

IKKβ,1μM

LOX,IC50:3.7μM

Quinone reductase 2,35nM-50nM

SIRT1,

SIRT2,

COX1,1.1 μM (cell free)

DNA polymerases α,3.3 μM (cell free)

DNA polymerases δ,5 μM (cell free)

实验溶液

0.5% CMC Na: 30 mg/mL

体外活性

Resveratrol inhibits cellular events associated with tumor initiation, promotion, and progression. Resveratrol inhibited the hydroperoxidase activity of COX-1 (ED50 = 3.7 μM) [1]. Resveratrol inhibits the activity of COX-1 and COX-2 (IC50s: 1.1 μM and 1.3 μM for COX-1 and COX-2, respectively) [2]. Resveratrol inhibited TPA-induced DNA binding of NF-kB. Moreover, resveratrol reduced the levels of p65/RelA, a functionally active subunit of NF-kB in nuclear fractions prepared from TPA-treated mouse skin. Resveratrol pretreatment significantly inhibited the phosphorylation of p65-(Ser-536) induced by TPA. Resveratrol pretreatment significantly attenuated TPA-induced stimulation of IKKa and IKKb activity [3]. DNA polymerase inhibiting activity of resveratrol was found to occur at 10 μM in an SV40 viral DNA replication assay. Resveratrol inhibits DNA polymerases α and δ (IC50s: 3.3 and 5 μM, respectively) [4].

体内活性

Resveratrol (RSV) treatment significantly upregulated eNOS gene expression and the levels of phosphorylated CREB in the aorta compared with the untreated control mice. Simultaneously, RSV treatment was observed to upregulate PKA activity and cGMP levels and inhibited superoxide generation in the aorta compared with untreated mice. Compared to vehicle-treated mice, chronic RSV treatment resulted in a significant improvement in endothelium-dependent vasorelaxation response to Ach. Treatment with RSV markedly attenuated the area of atherosclerotic lesions [5].

细胞实验

HAECs were treated with RSV in the presence of isobutylmethylxanthine (IBMX). The reaction was stopped by adding ice-cold 6% trichloroacetic acid and the supernatant fractions of the cellular lysates were extracted, dried and acetylated. Cyclic GMP levels were determined by an enzyme immunoassay kit and results standardized by protein levels [5].

细胞系: Human breast cancer cell MCF-7

动物实验

Eight-week male apoE-/- mice (C57BL/6 background) were used in the study. Mice were housed in photobiologic light-exposure chambers with a 12:12-h light:dark cycle, and eat food ad libitum. Mice were randomly divided into two groups: one group receiving a high cholesterol diet (HCD, 21% fat, 19.5% casein, and 1.5% cholesterol), the other receiving HCD supplemented with RSV (200 mg/100 g diet). All mice were fed for 8 weeks. To identify the potential role of PKA, some mice were treated with PKA specific inhibitor (KT5720, 1.2 mg/kg) via intraperitoneal injection once a day at the last 4 weeks. The animal experiments were conducted according to the institutional guidelines of Guangdong Provincial People’s Hospital [5].

动物模型:Human ovarian xenografts PA-1

化学信息

分子量

228.24

分子式

C14H12O3

CAS

501-36-0

溶解度

DMSO: 42 mg/mL (184 mM)

Ethanol: <1 mg/mL

Water: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

储存条件

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

备注

For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

配制溶液

1 mg 5 mg 10 mg
1 mM 4.381 ml 21.907 ml 43.814 ml
5 mM 0.876 ml 4.381 ml 8.763 ml
10 mM 0.438 ml 2.191 ml 4.381 ml
50 mM 0.088 ml 0.438 ml 0.876 ml
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