keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Resazurin sodium (Resazurin sodium salt) salt 是一种蓝色非荧光染料,广泛用作活细胞的代谢指示剂,通过被还原成荧光产物试卤灵,测量细菌和真核细胞活力。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 186 | 现货 | ||
1 g | ¥ 249 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 136 | 现货 |
产品描述 | Resazurin sodium (Resazurin sodium salt) salt is a blue non-fluorescent dye,is widely used as a metabolic indicator for living cells |
体外活性 | Resazurin inhibits the growth of a broad range of N. gonorrhoeae isolates, including those resistant to multiple antibiotics[1] |
体内活性 | In vivo resazurin did not limit the colonisation of mice with N. gonorrhoeae following vaginal infection.?The ineffectiveness of resazurin in vivo is likely due to its interaction with serum albumin, which completely diminishes its antimicrobial activity[1]. |
细胞实验 | Overnight broth cultures were used to inoculate FB containing resazurin sodium salt, resorufin sodium salt , resorufin acetate [Marker Gene Technologies, Eugene, OR; dissolved in dimethyl sulfoxide (DMSO)], resorufin butyrate or resorufin pentyl ether . In select experiments, FB was also supplemented with 3 g/dL bovine serum albumin (BSA) . Immediately following inoculation and 24 h later, cultures were serially diluted and were plated onto chocolate II agar. Plates were incubated at 37 °C in 5% CO2 and individual colonies were enumerated. The limit of detection was 100 CFU/mL[1]. |
别名 | Resazurin sodium salt, 刃天青钠, Diazoresorcinol (sodium) |
分子量 | 251.17 |
分子式 | C12H6NNaO4 |
CAS No. | 62758-13-8 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 150 mg/mL (597.21 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.9814 mL | 19.9068 mL | 39.8137 mL | 99.5342 mL |
5 mM | 0.7963 mL | 3.9814 mL | 7.9627 mL | 19.9068 mL | |
10 mM | 0.3981 mL | 1.9907 mL | 3.9814 mL | 9.9534 mL | |
20 mM | 0.1991 mL | 0.9953 mL | 1.9907 mL | 4.9767 mL | |
50 mM | 0.0796 mL | 0.3981 mL | 0.7963 mL | 1.9907 mL | |
100 mM | 0.0398 mL | 0.1991 mL | 0.3981 mL | 0.9953 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Resazurin sodium 62758-13-8 Microbiology/Virology Antibacterial inhibit Resazurin Resazurin sodium salt 刃天青钠 Inhibitor Bacterial Diazoresorcinol (sodium) Diazoresorcinol inhibitor