Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ROC-325 是一种有效的口服活性自噬抑制剂,具有抗癌活性。 ROC-325 诱导肾细胞癌凋亡并表现出良好的特异性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 397 | 现货 | ||
2 mg | ¥ 588 | 现货 | ||
5 mg | ¥ 842 | 现货 | ||
10 mg | ¥ 1,310 | 现货 | ||
25 mg | ¥ 2,620 | 现货 | ||
50 mg | ¥ 3,890 | 现货 | ||
100 mg | ¥ 5,580 | 现货 | ||
500 mg | ¥ 11,600 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 987 | 现货 |
产品描述 | ROC-325 is an effective and orally active inhibitor of autophagy with anticancer activity. ROC-325 induces renal cell carcinoma apoptosis and exhibits favorable selectivity. |
体外活性 | ROC-325 (5 μM) leads to the formation of LC3B punctae and a robust increase in LC3B levels in both A498 and 786-0 RCC cells. ROC-325 promotes a dose-dependent increase in LC3B expression in a manner that correlated with a corresponding increase in the levels of p62 and cathepsin D. ROC-325 inhibits cells growth with IC50 values of 4.9 μM, 11 μM, 4.6 μM, 5.4 μM, 7.4 μM, 11 μM, 8.2 μM, 5.8 μM, 5.0 μM, 11 μM, 8.4 μM and 6.0 μM for A498, A549, CFPAC-1, COLO-205, DLD-1, IGROV-1, MCF-7, MiaPaCa-2, NCI-H69, PC-3, RL and UACC-62 cells, respectively. ROC-325 induces the deacidification of lysosomes, accumulation of autophagosomes, and disrupted autophagic flux[1]. |
体内活性 | In mice bearing 786-0 RCC xenografts, ROC-325 (25, 40, and 50 mg/kg; oral) leads to significant, dose-dependent inhibition of disease progression and inhibits autophagy in vivo[1]. |
分子量 | 503.06 |
分子式 | C28H27ClN4OS |
CAS No. | 1859141-26-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 30 mg/mL (59.64 mM), Sonication is recommended.
H2O: 0.8 mg/mL (1.59 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 1.9878 mL | 9.9392 mL | 19.8783 mL | 49.6959 mL |
DMSO | 5 mM | 0.3976 mL | 1.9878 mL | 3.9757 mL | 9.9392 mL |
10 mM | 0.1988 mL | 0.9939 mL | 1.9878 mL | 4.9696 mL | |
20 mM | 0.0994 mL | 0.497 mL | 0.9939 mL | 2.4848 mL | |
50 mM | 0.0398 mL | 0.1988 mL | 0.3976 mL | 0.9939 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ROC-325 1859141-26-6 Apoptosis Autophagy anticancer inhibit ROC325 ROC 325 Autophagosomes cathepsin-D lysosomes oral Inhibitor inhibitor