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RN-1734

RN-1734

产品编号 T7572   CAS 946387-07-1
别名: 2,4-二氯-N-(异丙基)-N-[2-[(异丙基)氨基]乙基]苯磺酰胺

RN-1734是TRPV4通道的选择性拮抗剂,拮抗 4αPDD 介导的 TRPV4 活化,作用于 hTRPV4, mTRPV4, 和 rTRPV4的IC50分别为 2.3 μM、5.9 μM、3.2 μM。RN-1734能显著降低白细胞介素 1β和肿瘤坏死因子 α 的产生,而不改变 olig2- 阳性细胞的数量。

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RN-1734 Chemical Structure
RN-1734, CAS 946387-07-1
规格 价格/CNY 货期 数量
2 mg ¥ 253 现货
5 mg ¥ 455 现货
10 mg ¥ 638 现货
25 mg ¥ 1,280 现货
50 mg ¥ 1,930 现货
100 mg ¥ 2,880 现货
500 mg ¥ 6,250 现货
1 mL * 10 mM (in DMSO) ¥ 543 现货
产品目录号及名称: RN-1734 (T7572)
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纯度: 98%
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 RN-1734 is selective TRPV4 channel antagonist(IC50 of 2.3 μM,5.9 μM,3.2 μM for hTRPV4, mTRPV4 and rTRPV4,respectively)
靶点活性 hTRPV4:2.3 μM , rTRPV4:3.2 μM, mTRPV4:5.9 μM
体外活性 In vitro, RN-1734 treatment clearly inhibited the influx of calcium and decreased the levels of IL-1β and TNF-α in lipopolysaccharide (LPS)-activated microglial cells by suppressing NF-κB P65 phosphorylation.?Apoptosis of oligodendrocyte induced by LPS-activated microglia was also alleviated by RN-1734.?Activation of TRPV4 in microglia is involved in oligodendrocyte apoptosis through the activation of the NF-κB signaling pathway, thus revealing a new mechanism of CNS demyelination[1].
细胞实验 LPS-treated microglial cells were further divided into a LPS group, a vehicle (DMSO treatment) group and a RN-1734 group.?The cells were treated with 1 μg/ml LPS (Sigma) or RN-1734 (10 μM) for 3 h .?Next, the cells were triple-washed with DMEM medium to remove LPS, and then incubated in DMEM with or without RN1734 for 24 h. Finally, the cells were collected for TRPV4 immunocytostaining, and the supernatant was collected separately for enzyme-linked immunosorbent assay (ELISA) or for the oligodendrocyte apoptosis experiment.?The supernatant of the microglial cells was mixed with DMEM/F12 at a ratio of 1:1.?The mixed medium without LPS was used for the control (Ctrl) group, the medium with LPS only was used for the conditional medium (CM) group, and the media with LPS and DMSO or RN-1734 were used for the CM-vehicle group and the CM-RN1734 group, respectively.?After oligodendrocytes grew for 2 days in 6-well plates in DMEM/F12 containing 10% FBS, the differentiation medium was replaced with the described Ctrl or different CM media for 24 h. Then, the cells were harvested for apoptosis analysis and western blot analysis separately[1].
别名 2,4-二氯-N-(异丙基)-N-[2-[(异丙基)氨基]乙基]苯磺酰胺
分子量 353.31
分子式 C14H22Cl2N2O2S
CAS No. 946387-07-1

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 50 mg/mL (141.52 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8304 mL 14.1519 mL 28.3038 mL 70.7594 mL
5 mM 0.5661 mL 2.8304 mL 5.6608 mL 14.1519 mL
10 mM 0.283 mL 1.4152 mL 2.8304 mL 7.0759 mL
20 mM 0.1415 mL 0.7076 mL 1.4152 mL 3.538 mL
50 mM 0.0566 mL 0.283 mL 0.5661 mL 1.4152 mL
100 mM 0.0283 mL 0.1415 mL 0.283 mL 0.7076 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Meiying, Liu, Xuan, et al. TRPV4 Inhibition Improved Myelination and Reduced Glia Reactivity and Inflammation in a Cuprizone-Induced Mouse Model of Demyelination.[J]. Frontiers in cellular neuroscience, 2018. 2. Kato K , Morita I . Acidosis environment promotes osteoclast formation by acting on the last phase of preosteoclast differentiation: A study to elucidate the action points of acidosis and search for putative target molecules[J]. European Journal of Pharmacology, 2011, 663(1-3):27-39.
TRPV4 agonist-1 Oleoyl Serotonin ASP7663 Pinokalant GSK2798745 Methyl Kakuol (Z)-Capsaicin 1,4-Cineole

相关化合物库

该产品包含在如下化合物库中:
经典已知活性库 ReFRAME 相关化合物库 神经信号分子库 NO PAINS 化合物库 已知活性化合物库 抑制剂库 离子通道库 抗癌化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
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计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

RN-1734 946387-07-1 Membrane transporter/Ion channel TRP/TRPV Channel 2,4-二氯-N-(异丙基)-N-[2-[(异丙基)氨基]乙基]苯磺酰胺 Inhibitor Transient receptor potential channels TRP Channel RN 1734 RN1734 inhibit inhibitor

 

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