Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RK33 (RK 33) 是 DDX3(一种 RNA 解旋酶)的一流小分子抑制剂,可导致 G1 细胞周期停滞,诱导细胞凋亡,并促进 DDX3 过表达细胞的辐射增敏。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 356 | 现货 | ||
2 mg | ¥ 640 | 现货 | ||
5 mg | ¥ 1,150 | 现货 | ||
10 mg | ¥ 1,830 | 现货 | ||
25 mg | ¥ 3,830 | 现货 | ||
50 mg | ¥ 5,480 | 现货 | ||
100 mg | ¥ 7,690 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,150 | 现货 |
产品描述 | RK33 (RK 33) is a first-in-class small molecule inhibitor of DDX3 (a RNA helicase) and causes G1 cell cycle arrest, induces apoptosis, and promotes radiation sensitization in DDX3-overexpressing cells. |
体外活性 | RK-33 binds specifically to DDX3, but not to the closely related proteins DDX5 and DDX17. RK-33 inhibits cancer growth and radiosensitizes lung cancer cells in a DDX3-dependent manner. RK-33 has no effect on either mitochondrial respiration or ATP generation. RK-33 curbs proliferation and induces apoptosis in a DDX3-dependent fashion. Wnt signaling is mediated by DDX3 and inhibited by RK-33. RK-33 impairs radiation-induced DNA damage repair by inhibiting NHEJ activity[1]. |
体内活性 | RK-33 in combination with radiation induces tumor regression in multiple mouse models of lung cancer. RK-33, at the dose used, is non-toxic in SCID mice. RK-33-treated mice do not exhibit any discernable morphological changes[1]. |
细胞实验 | Healthy, 60-70% confluent MDA-MB-231 cells are transduced with shDDX3 lentivirus particles. Knockdown of DDX3 expression is confirmed both by qRT-PCR and immunoblotting. MDA-MB-231 cells are treated with RK-33 (7.5 μM) for 12 h and harvested for RNA. Microarray experiments are performed.(Only for Reference) |
别名 | RK-33, RK 33 |
分子量 | 428.44 |
分子式 | C23H20N6O3 |
CAS No. | 1070773-09-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 79 mg/mL (184.4 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.334 mL | 11.6702 mL | 23.3405 mL | 58.3512 mL |
5 mM | 0.4668 mL | 2.334 mL | 4.6681 mL | 11.6702 mL | |
10 mM | 0.2334 mL | 1.167 mL | 2.334 mL | 5.8351 mL | |
20 mM | 0.1167 mL | 0.5835 mL | 1.167 mL | 2.9176 mL | |
50 mM | 0.0467 mL | 0.2334 mL | 0.4668 mL | 1.167 mL | |
100 mM | 0.0233 mL | 0.1167 mL | 0.2334 mL | 0.5835 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
RK33 1070773-09-9 Immunology/Inflammation Neuroscience COX RK-33 RK 33 Inhibitor inhibitor inhibit