Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RG2833 (RGFP109) 是一种脑渗透性 HDAC 抑制剂,对 HDAC1 和 HDAC3 的 IC50 分别为 60 nM 和 50 nM,Ki 值分别为 32 和 5 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 148 | 现货 | ||
5 mg | ¥ 326 | 现货 | ||
10 mg | ¥ 592 | 现货 | ||
25 mg | ¥ 929 | 现货 | ||
50 mg | ¥ 1,230 | 现货 | ||
100 mg | ¥ 1,980 | 现货 | ||
200 mg | ¥ 3,490 | 现货 | ||
500 mg | ¥ 5,670 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 413 | 现货 |
产品描述 | RG2833 (RGFP109) (RGFP109), a brain-penetrant HDAC inhibitor, is with IC50 of 60 nM and 50 nM for HDAC1 and HDAC3, respectively. |
靶点活性 | HDAC1:32 nM(Ki), HDAC3:5 nM(Ki) |
体外活性 | In unstimulated peripheral blood mononuclear cells, RG2833 upregulates the level of the synaptic mRNA and protein in a dose-dependent manner. |
激酶实验 | Aconitase activities are determined by homogenization of mouse brain tissues on ice at 10% w/v in CellLytic MT Mammalian Tissue Lysis/Extraction buffer, followed by centrifugation at 800×g?for 10?min at 4°C. Tissue lysates (50?μL) are then added to 200?μL of substrate mix (50?mM Tris/HCl pH 7.4, 0.4?mM NADP, 5?mM Na citrate, 0.6?mM MgCl2, 0.1% (v/v) Triton X-100 and 1U isocitrate dehydrogenase) and the reactions are incubated at 37°C for 15?min, followed by spectrophotometric absorbance measurements every minute for 15?min at 340?nm 37°C to determine the reaction slope. Aconitase activities of mouse brain tissues are then normalized to citrate synthase activities, which are determined using a citrate synthase assay kit. |
别名 | RGFP109 |
分子量 | 339.43 |
分子式 | C20H25N3O2 |
CAS No. | 1215493-56-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 63 mg/mL (185.6 mM)
Ethanol: 10 mg/mL (29.46 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 2.9461 mL | 14.7306 mL | 29.4612 mL | 73.6529 mL |
5 mM | 0.5892 mL | 2.9461 mL | 5.8922 mL | 14.7306 mL | |
10 mM | 0.2946 mL | 1.4731 mL | 2.9461 mL | 7.3653 mL | |
20 mM | 0.1473 mL | 0.7365 mL | 1.4731 mL | 3.6826 mL | |
DMSO | 50 mM | 0.0589 mL | 0.2946 mL | 0.5892 mL | 1.4731 mL |
100 mM | 0.0295 mL | 0.1473 mL | 0.2946 mL | 0.7365 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
RG2833 1215493-56-3 Chromatin/Epigenetic DNA Damage/DNA Repair HDAC RGFP-109 Inhibitor RG 2833 RGFP109 inhibit RGFP 109 Histone deacetylases RG-2833 inhibitor