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Pyrrolidinedithiocarbamate ammonium

产品编号 T3147 CAS 5108-96-3

别名: PDTC, Pyrrolidinedithiocarbamate, 吡咯烷二硫代氨基甲酸铵, APDC, 1-Pyrrolidinedithiocarboxylic acid ammonium salt, Ammonium pyrrolidinedithiocarbamate, 吡咯烷二硫代甲酸铵

Pyrrolidinedithiocarbamate ammonium (1-Pyrrolidinedithiocarboxylic acid ammonium salt) 是一种选择性的、能透过血脑屏障的NF-κB 抑制剂。

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Pyrrolidinedithiocarbamate ammonium Chemical Structure

Pyrrolidinedithiocarbamate ammonium, CAS 5108-96-3

规格	价格/CNY	货期
100 mg	￥ 286	待询
200 mg	￥ 397	待询
500 mg	￥ 672	待询
1 mL * 10 mM (in DMSO)	￥ 185	待询

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其他形式的 Pyrrolidinedithiocarbamate ammonium:

(2R,4R)-APDC
询价

选择批次

纯度: 100%

纯度: 98%

更多批次查询请联系客服

生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

产品描述	Pyrrolidinedithiocarbamate ammonium (1-Pyrrolidinedithiocarboxylic acid ammonium salt), a selective NF-κB inhibitor, inhibits translation of nitric oxide synthase mRNA to prevent induction.
体外活性	Pretreatment of cells with PDTC (3-1000 mM) dose-dependently attenuate IL-8 production. Furthermore, PDTC (100 mM) suppresses the accumulation of IL-8 mRNA. PDTC inhibites the activation of NF-kB, because PDTC suppresses both NF-kB DNA binding and NF-kB-dependent transcriptional activity. Taken together, our data demonstrate that NF-kB inhibition with PDTC decrease IL-8 production by intestinal epithelial cells[1].
体内活性	The DSS+PDTC-treated groupⅡ exhibited suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels are significantly lower in DSS+PDTC-treated groupⅡ. These findings suggest that suppression of NF-κB activity by PDTC can delay the healing of mucosal tissue defects (erosions or ulcers) arising from inflammation, but that it can strongly suppress the expression of inf-lammatory cytokines (IL-1β and TNF-α), resulting in significant alleviation of colitis. PDTC is useful for the treatment of ulcerative colitis[2].
激酶实验	All binding studies are performed in an HTRF assay buffer consisting of dPBS supplemented with 0.1% (with v) bovine serum albumin and 0.05% (v/v) Tween-20. For the PD-l-Ig/PD-Ll-His binding assay, inhibitors are pre-incubated with PD-Ll-His (10 nM final) for 15 m in 4 μL of assay buffer, followed by addition of PD-l-Ig (20 nM final) in 1 μL of assay buffer and further incubation for 15 m. PD-L1 from either human, cyno, or mouse are used. HTRF detection is achieved using europium crypate-labeled anti- Ig (1 nM final) and allophycocyanin (APC) labeled anti-His (20 nM final). Antibodies are diluted in HTRF detection buffer and 5 μL is dispensed on top of binding reaction. The reaction mixture is allowed to equilibrate for 30 minutes and signal (665 nm/620 nm ratio) is obtained using an En Vision fluorometer. Additional binding assays are established between PD-1-Ig/PD-L2-His (20, 5 nM, respectively), CD80-His/PD-Ll-Ig (100, 10 nM, respectively) and CD80-His/CTLA4-Ig (10, 5 nM, respectively).
细胞实验	The human colon cancer cell line HT-29 is obtained and cells are grown in modified McCoy's 5A medium supplemented with 10% fetal bovine serum. To study the effect of PDTC on IL-8 production, HT-29 cells in 96-well plates are induced with 20 ng/mL of IL-1b for 18 h. Various concentrations (3-1000 mM) of PDTC or its vehicle (culture medium) are added to the cells 30 min prior to IL-1b stimulation. The concentration of IL-8 in the supernatant is determined using solid-phase enzyme-linked immunosorbent assay, as described previously employing the multiple antibody sandwich principle that specifically detects human IL-8[1].

别名	PDTC, Pyrrolidinedithiocarbamate, 吡咯烷二硫代氨基甲酸铵, APDC, 1-Pyrrolidinedithiocarboxylic acid ammonium salt, Ammonium pyrrolidinedithiocarbamate, 吡咯烷二硫代甲酸铵
分子量	164.29
分子式	C5H12N2S2
CAS No.	5108-96-3

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 16.4 mg/mL(100 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.0868 mL	30.434 mL	60.868 mL	152.1699 mL
	5 mM	1.2174 mL	6.0868 mL	12.1736 mL	30.434 mL
	10 mM	0.6087 mL	3.0434 mL	6.0868 mL	15.217 mL
	20 mM	0.3043 mL	1.5217 mL	3.0434 mL	7.6085 mL
	50 mM	0.1217 mL	0.6087 mL	1.2174 mL	3.0434 mL
	100 mM	0.0609 mL	0.3043 mL	0.6087 mL	1.5217 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

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浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

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配液浓度

参考文献

1. Qin, J., Cao, Z., Li, X., Kang, X., Xue, Y., & Li, Y. et al. (2014). Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-κB activation and CYP2E1 content of rats with immunological liver injury. Pharmaceutical Biology, 52(11), 1460-1466. doi: 10.3109/13880209.2014.898075 2. Gao Y, Elamin E, Zhou R, et al. FKBP51 promotes migration and invasion of papillary thyroid carcinoma through NF‑κB‑dependent epithelial‑to‑mesenchymal transition[J]. Oncology letters. 2018 Dec;16(6):7020-7028. 3. Lu S, Luo Y, Sun G B, et al. Ginsenoside compound K attenuates ox-LDL-mediated macrophage inflammation and foam cell formation via autophagy induction and modulating NF-κB, p38, and JNK MAPK signaling[J]. Frontiers in Pharmacology. 2020, 11. 4. Zhao L, Xia M, Wang K, et al. A Long Non-coding RNA IVRPIE Promotes Host Antiviral Immune Responses Through Regulating Interferon β1 and ISG Expression[J]. Frontiers in Microbiology. 2020, 11: 260. 5. Shi F1, Deng Z1, Zhou Z2, Jiang CY1, Zhao RZ1, Sun F1,3, Cui D1,3, Bei XY1,3, Yang BY1, Sun Q2, Wang XJ1,3, Wu Q2, Xia SJ1,3, Han BM1,3. QKI‐6 inhibits bladder cancer malignant behaviours through down‐regulating E2F3 and NF‐κB signalling. Journal of cellular and molecular medicine. 6. Li Y, Niu M, Zhao A, et al. CXCL12 is involved in α-synuclein-triggered neuroinflammation of Parkinson’s disease[J]. Journal of Neuroinflammation. 2019, 16(1): 1-14.

文献引用

1. Zhu T, Chen X, Qiu H, et al. Aspirin Alleviates Particulate Matter Induced Asymptomatic Orchitis of Mice via Suppression of cGAS-STING Signaling. Frontiers in immunology. 2021, 12. 2. Guo T, Liu C, Yang C, et al. Immunoproteasome subunit PSMB8 regulates microglia-mediated neuroinflammation upon manganese exposure by PERK signaling. Food and Chemical Toxicology. 2022: 112951. 3. Li Y, Niu M, Zhao A, et al. CXCL12 is involved in α-synuclein-triggered neuroinflammation of Parkinson’s disease. Journal of Neuroinflammation. 2019, 16(1): 1-14 4. Shi F1, Deng Z1, Zhou Z2, Jiang CY1, Zhao RZ1, Sun F1,3, Cui D1,3, Bei XY1,3, Yang BY1, Sun Q2, Wang XJ1,3, Wu Q2, Xia SJ1,3, Han BM1,3. QKI‐6 inhibits bladder cancer malignant behaviours through down‐regulating E2F3 and NF‐κB signalling. Journal of Cellular and Molecular Medicine. 2019, 23(10): 6578-6594 5. Zhao L, Xia M, Wang K, et al. A Long Non-coding RNA IVRPIE Promotes Host Antiviral Immune Responses Through Regulating Interferon β1 and ISG Expression. Frontiers in Microbiology. 2020, 11: 260 6. Lu S, Luo Y, Sun G B, et al. Ginsenoside compound K attenuates ox-LDL-mediated macrophage inflammation and foam cell formation via autophagy induction and modulating NF-κB, p38, and JNK MAPK signaling. Frontiers in Pharmacology. 2020 Sep 15;11:567238 7. Wang Y, Gao W. Effects of TNF-α on autophagy of rheumatoid arthritis fibroblast-like synoviocytes and regulation of the NF-κB signaling pathway. Immunobiology. 2021, 226(2): 152059. 8. Gao Y, Elamin E, Zhou R, et al. FKBP51 promotes migration and invasion of papillary thyroid carcinoma through NF‑κB‑dependent epithelial‑to‑mesenchymal transition. Oncology letters. 2018 Dec;16(6):7020-7028. 9. Tian X, Liu F, Wang Z, et al.Modified Biejia Jianwan decoction restrains PD-L1-mediated immune evasion through the HIF-1α/STAT3/NF-κB signaling pathway.Journal of Ethnopharmacology.2023: 117577.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Pyrrolidinedithiocarbamate ammonium 5108-96-3 NF-Κb NF-κB TNF-α Inhibitor 1-Pyrrolidinedithiocarboxylic acid ammonium PDTC Nuclear factor-κB inhibit Pyrrolidinedithiocarbamate 吡咯烷二硫代氨基甲酸铵 APDC 1-Pyrrolidinedithiocarboxylic acid ammonium salt ulcerative Ammonium pyrrolidinedithiocarbamate inflammation 吡咯烷二硫代甲酸铵 IL-1β colitis Nuclear factor-kappaB inhibitor

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Pyrrolidinedithiocarbamate ammonium

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

文献引用

相关产品

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剂量换算

体内实验配液计算器

技术支持

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