Protocatechuic acid

产品编号: T0562 别名:

3, 4-Dihydroxybenzoic acid,Protocatechuate,3,4-二羟基苯甲酸,3,4-Dihydroxybenzoic acid,原儿茶酸

Protocatechuic acid 是天然的多酚类物质，具有神经保护活性。

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CAS：99-50-3

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生物活性化学信息储存和溶解度信息参考文献引用文献

产品描述

Protocatechuic acid (3, 4-dihydroxybenzoic acid) is a natural phenolic compound found in many edible and medicinal plants. Recent studies indicate that it could be used as a protective agent against cardiovascular diseases and neoplasms. The mechanism of its action is mostly associated with antioxidant activity, including inhibition of generation as well as scavenging of free radicals and up-regulating enzymes which participate in their neutralization.

体外活性

Protocatechuic acid inhibits the aggregation of Aβ and αS and destabilizes their preformed fibrils that prevent the death of PC12 cells triggered by Aβ- and αS-induced toxicity[3].

体内活性

Protocatechuic acid can prevent stress-induced immobility time in forced swim test without altering locomotor activity in mice. In addition, Protocatechuic acid treatment attenuates the elevation of serum corticosterone, lipid peroxidation and restores enzymatic antioxidants in cerebral cortex and hippocampus in ARS mice[1]. Rat administered cadmium and treated with prostigmine and doses of Protocatechuic acid (10−20 mg/kg) has significantly reduced BChE activity. Cadmium and either prostigmine or Protocatechuic acid (10−20 mg/kg) treated rats shows a marked reduction in MDA level[2].

激酶实验

AChE activity investigation is carried out in a reaction mixture containing 50 μL of tissue homogenate, 50 μL of 5, 5'-dithiobis-(2-nitrobenzoic) acid (DTNB), 1175 μL of 0.1 M phosphate-buffered solution, pH 8.0. After incubation for 20 min at 25°C, 25 μL of acetylthiocholine iodide solution is added as the substrate. The AChE activity is determined as changes in absorbance reading at 412 nm for 3 min at 25°C and using a UV/Visible spectrophotometer.

细胞实验

Protocatechuic acid is dissolved in DMSO. Dilutions of Protocatechuic acid (2, 5, 10, 20, 50, and 100 μM) are prepared from stock solutions, with serum-free culture medium. Equal volumes of each solution are mixed with Aβ1-42 (10 μM), then incubated for 24 h on a thermoblock, with continuous agitation, and then exposed to PC12 cells for 24 h to test whether Protocatechuic acid can prevent cell death triggered by Aβ. Cell viability is determined by MTT reduction assay. Cells are treated with 200 μL per well of MTT solution (final concentration, 0.5 mg/mL in DMEM-Glutamax medium) for 3 h, at 37°C, with 5% CO2. The dark blue formazan crystals that formed are solubilized with 100 μL per well of DMSO, for 30 min. Absorbance is measured at 540 nm, with a microplate reader. Results are expressed as the percentage of MTT reduction in relation to the absorbance of control cells at 100%.

Cas No.

99-50-3

分子式

C7H6O4

分子量

154.121

别名

3, 4-Dihydroxybenzoic acid;Protocatechuate;3,4-二羟基苯甲酸;3,4-Dihydroxybenzoic acid;原儿茶酸;Protocatechuic acid

参考文献

[1]Jang S A, Hwang Y H, Kim T, et al. Anti-Osteoporotic and Anti-Adipogenic Effects of the Water Extract of Drynaria roosii Nakaike in Ovariectomized Mice Fed a High-Fat Diet[J]. Molecules. 2019, 24(17): 3051.[2]Dong Q, Li Y, Liu G, et al. High oxygen treatments enhance the contents of phenolic compound and ganoderic acid, antioxidant and DNA damage protective activities of Ganoderma lingzhi fruiting body[J]. Frontiers in Microbiology. 2019, 10: 2363.[3]Hornedo-Ortega R , álvarez-Fernández, María Antonia, Cerezo, Ana Belén, et al. Protocatechuic acid: inhibition of fibril formation, destabilization of pre-formed fibrils of Amyloid-β and α-Synuclein, and neuroprotection[J]. Journal of Agricultural and Food Chemistry, 2016:acs.jafc.6b03217.[4]Chen Q, Sun D, Fang T, et al. In vitro allelopathic effects of compounds from Cerbera manghas L. on three Dinophyta species responsible for harmful common red tides. Science of The Total Environment. 2020: 142253.[5]Adefegha SA, et al. Alterations of Na+/K+-ATPase, cholinergic and antioxidant enzymes activity by protocatechuic acid in cadmium-induced neurotoxicity and oxidative stress in Wistar rats. Biomed Pharmacother. 2016 Oct;83:559-568.[6]Thakare VN, et al. Attenuation of acute restraint stress-induced depressive like behavior and hippocampal alterations with protocatechuic acid treatment in mice. Metab Brain Dis. 2016 Oct 26.

引用文献

[1]Chen Z, Yuan Y, Yang D, et al. Antiviral activities of Polygonum Perfoliatum L. extract and related phenolic acid constituents against hepatitis B virus. Journal of Medical Virology. 2022 [2]Dong Q, Li Y, Liu G, et al. High oxygen treatments enhance the contents of phenolic compound and ganoderic acid, antioxidant and DNA damage protective activities of Ganoderma lingzhi fruiting body. Frontiers in Microbiology. 2019, 10: 2363 [3]Tsang C C, Tang J Y M, Ye H, et al. Rare/cryptic Aspergillus species infections and importance of antifungal susceptibility testing. Mycoses. 2020, 63(12): 1283-1298 [4]Jang S A, Hwang Y H, Kim T, et al. Anti-Osteoporotic and Anti-Adipogenic Effects of the Water Extract of Drynaria roosii Nakaike in Ovariectomized Mice Fed a High-Fat Diet. Molecules. 2019, 24(17): 3051

储存和溶解度

DMSO:10 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years

剂量换算 Protocatechuic acid 99-50-3

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工作液浓度: mg/ml;

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体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

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Keywords

Endogenous Metabolite Inhibitor 3, 4-Dihydroxybenzoic acid Protocatechuate 3,4-二羟基苯甲酸 Protocatechuic acid 3,4-Dihydroxybenzoic acid 原儿茶酸 inhibit 99-50-3

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Protocatechuic acid

3, 4-Dihydroxybenzoic acid,Protocatechuate,3,4-二羟基苯甲酸,3,4-Dihydroxybenzoic acid,原儿茶酸

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