Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ponicidin (Rubescensine B) 是来源于冬凌草的一种二萜,具有免疫调节、抗炎、抗病毒和抗癌作用。它可诱导胃癌细胞凋亡,降低 JAK2 和 STAT3 的磷酸化水平。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 568 | 现货 | ||
5 mg | ¥ 1,490 | 现货 | ||
10 mg | ¥ 2,190 | 现货 | ||
25 mg | ¥ 3,730 | 现货 | ||
50 mg | 待询 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,190 | 现货 |
产品描述 | Ponicidin (Rubescensine B) is a diterpenoid derived from Rabdosia rubescens. Ponicidin exhibits immunoregulatory, anti-inflammatory, anti-viral, and anti-cancer activity. Ponicidin induces apoptosis of gastric carcinoma cells, decreases the phosphorylation of JAK2 and STAT3, and shows no effect on protein levels of JAK2 and STAT3. |
体外活性 | K562 cells in culture medium in vitro were given different concentrations of Ponicidin (10-50 micromol x L(-1)) for 24, 48, and 72 h. The inhibitory rate of the cells was measured by MTT assay, cell apoptotic rates were detected by flow cytometry (FCM) using Annexin V staining after K562 cells were treated with different concentrations of Ponicidin for 72 hours, and cell morphology was observed by Wright-Giemsa staining. Ponicidin (over 30 micromol x L(-1)) could inhibit the growth of K562 cells in both time- and dose-dependent manner. FCM analysis revealed that apoptotic cells were gradually increased in a dose-dependent manner after treatment for 72 hours, and that marked morphological changes of cell apoptosis such as condensation of chromatin was clearly observed by Wright-Giemsa staining after treatment by 50 micromol x L(-1) Ponicidin. Furthermore, Western blotting also showed that expression of p-AKT and p-P85 in PI3K/AKT signaling pathways was downregulated dramatically whereas the expression of p-P38, as well as p-ERK and p-JNK, remained unchanged after the cells were treated by PON for 48 h. |
别名 | 冬凌草乙素, Rubescensine B |
分子量 | 362.42 |
分子式 | C20H26O6 |
CAS No. | 52617-37-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 250 mg/mL (689.81 mM), sonification is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.7592 mL | 13.7961 mL | 27.5923 mL | 68.9807 mL |
5 mM | 0.5518 mL | 2.7592 mL | 5.5185 mL | 13.7961 mL | |
10 mM | 0.2759 mL | 1.3796 mL | 2.7592 mL | 6.8981 mL | |
20 mM | 0.138 mL | 0.6898 mL | 1.3796 mL | 3.449 mL | |
50 mM | 0.0552 mL | 0.2759 mL | 0.5518 mL | 1.3796 mL | |
100 mM | 0.0276 mL | 0.138 mL | 0.2759 mL | 0.6898 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
冬凌草 | Rabdosia rubescens( Hemsl.)Hara | 微寒 | 苦, 甘 | 肺, 胃, 肝 |
鲁山香茶菜 | Rabdosiaamethystoides(Benth.)Hara | 凉 | 辛, 苦 | 肝, 肾 |
毛叶香茶菜 | Isodon japonicus (N. Burman) H. Hara | - | ― | |
水流豆 | Pongamia pinnata (Linn.) Merr. | 寒 | 苦 | 肺 |
显脉香茶菜 | Rabdosia nervosa (Benth.) Hara | 寒 | 微辛, 苦 |
中成药名称 | 处方组成 | 主治疾病 | 中成药类型 |
冬凌草糖浆 | 冬凌草 | 口服,一次10~20毫升,一日2次。 | 清热药 |
咽康含片 | 冬凌草,玄参,麦冬,桔梗,甘草,薄荷脑,冰片,艾纳香油,薄荷脑,冰片 | 含服,一次1片,每隔1~2小时1次。 | 清热药 |
冬凌草片 | 冬凌草 | 口服,一次2~5片,一日3次。 | 清热药 |
冬凌草胶囊 | 冬凌草 | 口服,一次2~5粒,一日3次。 | 清热药 |
咽康滴丸 | 冬凌草,玄参,麦冬,桔梗,薄荷脑,冰片,甘草,艾纳香油 | 含服,一次1滴,每隔1~2小时1次。 | 清热药 |
复方冬凌草含片 | 冬凌草,薄荷,桔梗,甘草 | 含服,一次1~2片,每小时1~2片,每天10~20片。 | 化痰、止咳、平喘药 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Ponicidin 52617-37-5 Angiogenesis Apoptosis Chromatin/Epigenetic JAK/STAT signaling Stem Cells JAK STAT inhibit Inhibitor 冬凌草乙素 Rubescensine B inhibitor