Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Pentamidine isethionate (Pentamidine diisethionate) 是抗微生物剂,会干扰 DNA 的生物合成。它抑制寄生虫Leishmania infantum,IC50为 2.5 μM。它是选择性蛋白酪氨酸磷酸酶和再生肝磷酸酶抑制剂。它有抗肿瘤和抗菌活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 249 | 现货 | ||
100 mg | ¥ 333 | 现货 | ||
500 mg | ¥ 772 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 274 | 现货 |
产品描述 | Pentamidine isethionate (Pentamidine diisethionate) is a synthetic amidine derivative, Pentamidine Isethionate is an antiprotozoal and antifungal agent. |
靶点活性 | Leishmania infantum:2.5 μM (IC50) |
体外活性 | Pentamidine is known experimentally to interfere with numerous cellular processes. Specifically, Pentamidine has been shown to bind to DNA in a nonintercalative manner and appears to preferentially bind to kinetoplast DNA in trypanosomes. Additionally, Pentamidine may inhibit RNA polymerase and ribosomal function, as well as nucleic acid, protein, phospholipid, and polyamine synthesis. Pentamidine also inhibits certain proteases, including trypsin, and impairs cellular oxygen consumption. [1] Pentamidine has a potent in vitro antiprotozoal activity. Pentamidine displays cytotoxic activity against L. infantum promastigotes with IC50 of 2.5 μM. 2.5 μM Pentamidine induces early programmed cell death in 49.6% of L. infantum promastigotes. 2.5 μM Pentamidine induces a notorious decrease in promastigotes in both G1 and S phases relative to the control-untreated samples (G1:77.0 vs 15.0%; S:11.0 vs 2.4% for control- and pentamidine-treated promastigotes, resp). Pentamidine is able to bind with calf-thymus DNA (CT-DNA) and induces conformational changes in the DNA double helix. Pentamidine also binds with ubiquitin to modifiy the β-cluster of ubiquitin. [2] Pentamidine is an inhibitor of phosphatase of regenerating liver (PRLs). 1 μg/mL of Pentamidine complete inhibits the activity of recombinant PTP1B in dephosphorylating a phos-photyrosine peptide. 10 μg/mL of Pentamidine completely inhibits the activities of recombinant PRL-1, PRL-2 and PRL-3 in dephosphorylating a phosphotyrosine peptide substrate. Incubation with Pentamidine (1 μg/mL) for 48 h reduces the activity of intracellular PRL phosphatases in transfected NIH3T3 cells by more than 85%. 10 μg/mL Pentamidine completely inhibits the growth of melanoma cell line (WM9), prostate carcinoma cell line (DU145 and C4–2), ovarian carcinoma cell line (Hey), colon carcinoma cell line (WM480), and lung carcinoma cell line (A549) which all express endogenous PRLs. [3] |
体内活性 | Pentamidine has a potent antiprotozoal activity in animal models. Pentamidine (0.3-9 mg/L) decreased the viability of P. carinii in experimental models in chick embryo lung epithelial cells and lung cells of rats with pneumonia. 5 mg/kg Pentamidine treatment for 2 weeks eradicates Pneumocystis carinii pneumonia in 75% of the animals. [4] Pentamidine inhibits the growth of WM9 human melanoma tumors in nude mice. During the 16-week study period, the tumors in 250 μg pentamidine-treated mice stays at sizes similar to those at the treatment initiation point, whereas the tumors in the control mice grow so rapidly that humane sacrifice of the animals is required at the 4th week. Pentamidine induces significant necrosis that accounts for more than 50% of the tumor mass. [3] |
激酶实验 | In vitro PTPase assays: Individual PTPases (0.01 μg/reaction) in 50 μL of PTPase buffer [50 mM Tris (pH 7.4)] are incubated at 22 ℃ for 10 min or as indicated in the absence or presence of inhibitory compounds. Substrates (0.2 mM phosphotyrosine peptide) are then added and allows to react at 22 ℃ for 18 hr. PTPase activity of individual reactions is measured by adding 100 μL of malachite green solution (UBI) and then quantifying the amounts of free phosphate cleaved by the PTPase from the peptide substrate by spectrometry (A660 nm). Relative PTPase activities are calculated based on the formula [(PTPase activity in the presence of an inhibitory compound)/(PTPase activity in the absence of the compound) × 100%]. Reactions performed under comparable conditions in the absence of recombinant PTPases only are used as controls and shows no detectable PTPase activity. |
细胞实验 | Cells are washed in 10% FCS contained RPMI 1640 medium twice, resuspended in 10% FCS medium, incubated at 37 ℃ for 16 hr, and then cultured at 37 ℃ in 10% FCS medium containing various amounts of Pentamidine for 6 days. The cell numbers in proliferation assays are determined by an MTT assay.(Only for Reference) |
别名 | Pentamidine isethionate salt, Pentamidine diisethionate, 喷他脒羟乙磺酸盐 |
分子量 | 592.68 |
分子式 | C23H36N4O10S2 |
CAS No. | 140-64-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 168.7 mM
H2O: 168.7 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 1.6873 mL | 8.4363 mL | 16.8725 mL | 42.1813 mL |
5 mM | 0.3375 mL | 1.6873 mL | 3.3745 mL | 8.4363 mL | |
10 mM | 0.1687 mL | 0.8436 mL | 1.6873 mL | 4.2181 mL | |
20 mM | 0.0844 mL | 0.4218 mL | 0.8436 mL | 2.1091 mL | |
50 mM | 0.0337 mL | 0.1687 mL | 0.3375 mL | 0.8436 mL | |
100 mM | 0.0169 mL | 0.0844 mL | 0.1687 mL | 0.4218 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Pentamidine isethionate 140-64-7 Metabolism Microbiology/Virology Phosphatase Antibacterial Antibiotic Parasite Antifungal DNA cell-cycle Pentamidine isethionate salt Bacterial pneumonia Pneumocystis PTP1B carinii biosynthetics Kidney MP601205 MP 601205 Pentamidine diisethionate arrest Pentamidine antitumor Fungal Inhibitor MP-601205 inhibit 喷他脒羟乙磺酸盐 inhibitor