Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Pamidronic acid 可用于各种骨吸收类疾病的研究。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 198 | 现货 | ||
25 mg | ¥ 313 | 现货 | ||
50 mg | ¥ 497 | 现货 | ||
100 mg | ¥ 797 | 现货 |
产品描述 | Pamidronic acid Leads to Bone Necrosis via Suppression of Wnt/β-Catenin Signaling in Human Bone Marrow Mesenchymal Stem Cells |
体外活性 | Pamidronate disodium inhibited Wnt and β-catenin signaling, which controls osteogenic differentiation in BMMSCs. Wnt3a, a Wnt and β-catenin signaling activator, reversed the negative effects caused by pamidronate disodium to salvage the osteogenic defect in BMMSCs[1]. |
细胞实验 | Primary human BMMSCs were isolated from the mandible and marrow tissue. A proliferation assay was performed to determine the experimental concentration of pamidronate disodium. Alkaline phosphatase (ALP) activity, ALP staining, and Alizarin red S (ARS) staining were assessed after treatment with pamidronate disodium (0, 0.1, 0.5, 1, 5, 10 μg/mL). Quantitative real-time polymerase chain reaction and western blotting specific for Wnt and β-catenin signaling genes or proteins were performed after treatment with pamidronate disodium 0.5 μg/mL. Wnt3a was used to observe the osteogenic differentiation of BMMSCs during treatment with pamidronate disodium 0.5 μg/mL[1]. |
分子量 | 235.07 |
分子式 | C3H11NO7P2 |
CAS No. | 40391-99-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 6 mg/mL (25.52 mM), sonification/heating is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 4.2541 mL | 21.2703 mL | 42.5405 mL | 106.3513 mL |
5 mM | 0.8508 mL | 4.2541 mL | 8.5081 mL | 21.2703 mL | |
10 mM | 0.4254 mL | 2.127 mL | 4.2541 mL | 10.6351 mL | |
20 mM | 0.2127 mL | 1.0635 mL | 2.127 mL | 5.3176 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Pamidronic acid 40391-99-9 Cytoskeletal Signaling Stem Cells Wnt/beta-catenin inhibit Inhibitor β-catenin Beta catenin Wnt inhibitor