Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Palbociclib (PD 0332991) 是一种 CDK 抑制剂,抑制 CDK4 和 CDK6 (IC50=11/16 nM),具有口服活性。Palbociclib 具有抗肿瘤活性,有用于 ER 阳性和 HER2 阴性乳腺癌的研究潜力。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 398 | 现货 | ||
10 mg | ¥ 519 | 现货 | ||
50 mg | ¥ 754 | 现货 | ||
100 mg | ¥ 869 | 现货 |
产品描述 | Palbociclib (PD 0332991) is a CDK inhibitor that inhibits CDK4 and CDK6 (IC50=11/16 nM) and is orally active. Palbociclib has anti-tumorigenic activity and has investigational potential for use in ER-positive and HER2-negative breast cancer. |
靶点活性 | CDK4:11 nM, CDK6:16 nM |
体外活性 |
方法:恶性横纹肌样瘤细胞 G401、MP-MRT-AN (AN)、KP-MRT-RY (RY)、KP-MRT-NS (NS) 和 KP-MRT-YM (YM) 用 Palbociclib (0-1 mM) 处理 24 h,使用 WST 方法检测细胞活力。 结果:Palbociclib 以浓度依赖性的方式有效抑制 AN、RY、G401 和 NS 细胞系,IC50 分别为 0.01 μM、0.01 μM,0.06 μM 和 0.6 μM。相反,YM 细胞系对 Palbociclib 具有抗性,IC50>10 μM。[1] 方法:人乳腺癌细胞 MDA-MB-453 用 Palbociclib (0.02-10 μmol/L) 处理 24 h,使用 Flow Cytometry 方法检测细胞周期情况。 结果:Palbociclib 导致 G1 期阻滞。[2] |
体内活性 |
方法:为检测体内抗肿瘤活性,将 Palbociclib (12.5-150 mg/kg,sodium lactate buffer (50 mmol/L, pH 4.0)) 口服给药给携带肿瘤 Colo-205 或 MDA-MB-435 的免疫缺陷小鼠,每天一次,持续十四天。 结果:Palbociclib 对多种人类肿瘤异种移植物模型显示出显著的抗肿瘤功效。对 Colo-205 肿瘤,PD 0332991 给药 14 天,可产生快速的肿瘤消退。[2] 方法:为检测体内抗肿瘤活性,将 Palbociclib (150 mg/kg,sodium lactate buffer (50 mM, pH = 4.0)) 灌胃给药给携带食道鳞状细胞癌 (ESCC) 肿瘤 EC109 或 KYSE150 的 nu/nu BALB/c 小鼠,每天一次,持续两周。 结果:Palbociclib 有效抑制 ESCC 肿瘤生长和肺转移。[3] |
激酶实验 | CDK assays are performed in 96-well filter plates. All CDK-cyclin kinase complexes are expressed in insect cells through baculovirus infection and purified. The substrate for the assays is a fragment (amino acids 792-928) of pRb fused to GST (GST·RB-Cterm). The total volume in each well is 0.1 mL containing a final concentration of 20 mM Tris-HCl, pH 7.4, 50 mM NaCl, 1 mM dithiothreitol, 10 mM MgCl2, 25 μM ATP (for CDK4-cyclin D1, CDK6-cyclin D2, and CDK6-cyclin D3) or 12 μM ATP (for CDK2-cyclin E, CDK2-cyclin A, and CDC2-cyclin B) containing 0.25 μCi of [γ-32P]ATP, 20 ng of enzyme, 1 μg of GST·RB-Cterm, and Palbociclib (0.001-0.1 μM). All components except the [γ-32P]ATP are added to the wells, and the plate is placed on a plate mixer for 2 min. The reaction is started by adding the [γ-32P]ATP and the plate is incubated at 25°C for 15 min. The reaction is terminated by addition of 0.1 mL of 20% trichloroacetic acid and the plate is kept at 4°C for at least 1 hour to allow the substrate to precipitate. The wells are then washed 5 times with 0.2 mL of 10% trichloroacetic acid and radioactive incorporation is determined with a β plate counter. |
细胞实验 | Palbociclib (PD) is prepared in DMSO and stored (?80°C), and then diluted with appropriate media before use[1]. MRT cell lines, G401, MP-MRT-AN (AN), KP-MRT-RY (RY), KP-MRT-NS (NS), and KP-MRT-YM (YM) cell lines are seeded in normal growth medium into 96-well cell plates. After 24 h, the culture medium is replaced with culture medium containing Palbociclib (0.05 or 1 μM) or DMSO. Cells are cultured and treated in triplicate. Cell proliferation is determined 8 days after the treatment by WST-8 assay using a Cell Counting Kit-8. |
别名 | PD 0332991, 帕博西尼, 帕布昔利布 |
化合物与蛋白结合的复合物 |
The X-ray co-crystal structure of human CDK6 and Palbociclib. |
分子量 | 447.53 |
分子式 | C24H29N7O2 |
CAS No. | 571190-30-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 1.3 mg/mL, Sonification is recommended.
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Palbociclib 571190-30-2 Cell Cycle/Checkpoint CDK PD 0332991 PD-0332991 estrogen receptor HER2 HCC inhibit 帕博西尼 Inhibitor Cyclin dependent kinase hepatocellular carcinoma cell cycle PD0332991 Breast cancer 帕布昔利布 inhibitor