Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PY-60 有效激活针对膜联蛋白 A2 (Kd = 1.4 µM) 的 YAP 转录活性。 PY-60 在能够通过增殖重塑表皮的成年动物中激活促增殖、依赖于 YAP 的转录程序。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 1,160 | 现货 | ||
5 mg | ¥ 2,790 | 现货 | ||
10 mg | ¥ 4,150 | 现货 | ||
25 mg | ¥ 6,630 | 现货 | ||
50 mg | ¥ 8,850 | 现货 | ||
100 mg | ¥ 11,900 | 现货 | ||
500 mg | ¥ 24,300 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 2,890 | 现货 |
产品描述 | PY-60 potently activates YAP transcriptional activities targeting annexin A2 (Kd = 1.4 µM). PY-60 activates a proproliferative, YAP-dependent transcriptional program in the adult animal capable of remodeling the epidermis through proliferation. |
靶点活性 | ANXA2:1.4 µM |
体外活性 | PY-60 robustly increased the levels of YAP-controlled transcripts (that is, ANRKD1, CYR61 and CTGF) in 293A cells and other human cell lines (that is, MCF10A, HEK293T, H69 and HaCaT), but did not augment the levels of YAP itself (YAP1). PY-60 induced luciferase activity in 293A-TEAD-LUC cells in the presence or absence of serum when cells were plated at high cell density with EC50s of 1.5 and 1.6 µM, respectively. PY-60 also promoted the association of YAP and TEAD proteins in cells in a dose-dependent manner and induced the nuclear localization of YAP in response to increased cell density[1]. |
体内活性 | PY-60 (10 uM) promoted a dramatic expansion of keratinocytes and K14-positive precursors. PY-60 resulted in an approximate doubling of epidermal thickness, a result derived from an increased number of keratinocytes per unit length of skin[1]. |
分子量 | 313.37 |
分子式 | C16H15N3O2S |
CAS No. | 2765218-56-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 95 mg/mL (303.16 mM), Sonification is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.1911 mL | 15.9556 mL | 31.9112 mL | 79.7779 mL |
5 mM | 0.6382 mL | 3.1911 mL | 6.3822 mL | 15.9556 mL | |
10 mM | 0.3191 mL | 1.5956 mL | 3.1911 mL | 7.9778 mL | |
20 mM | 0.1596 mL | 0.7978 mL | 1.5956 mL | 3.9889 mL | |
50 mM | 0.0638 mL | 0.3191 mL | 0.6382 mL | 1.5956 mL | |
100 mM | 0.0319 mL | 0.1596 mL | 0.3191 mL | 0.7978 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
PY-60 2765218-56-0 GPCR/G Protein Stem Cells Annexin A YAP PY60 PY 60 Inhibitor inhibitor inhibit