首页 GPCR/G Protein Histamine Receptor Osthole
Osthole
编号 T2848     别名: Osthol, Ostol, NSC 31868
CAS 484-12-8     分子式 C15H16O3     分子量 244.29
靶点: H1 receptor;
Osthole, a potential inhibitor of histamine H1 receptor, has been shown to stimulate osteoblast proliferation and differentiation.
选择批次  
规格 库存 单价 数量
200 mg 上海现货 344.00
1 mL * 10 mM (in DMSO) 上海现货 417.00
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生物活性

产品描述

Osthole, a potential inhibitor of histamine H1 receptor, has been shown to stimulate osteoblast proliferation and differentiation.

靶点活性

H1 receptor,

体外活性

在洗涤过的兔血小板中, Osthol能够抑制ADP,花生四烯酸,PAF,胶原蛋白,离子载体A23187和凝血酶诱导的血小板聚集和ATP释放。在人成骨细胞中,Osthole通过骨形态发生蛋白-2 / p38和细胞外信号调节激酶1/2通路,介导细胞分化。在细胞培养中,Osthole通过增加HBsAg的糖基化,抑制乙型肝炎病毒的分泌。

体内活性

Osthole在不同的动物中的LD50分别是,大鼠>125 mg/kg(i.v.);兔子>35 mg/kg(i.v.);狗>150 mg/kg(i.v.).

细胞实验

Osthole is dissolved in 96% ethyl alcohol and then sterilized through a 0.22 μg/mL filter and stored at 4°C as stock solutions for later dilution[1]. Peripheral blood samples are collected from participants between 7.00 and 9.00 a.m. on the first study day and these are concentrated in grouping tubes with K3EDTA. Fresh PBMCs are then prepared. Isolated cells are seeded on 24-well plates at 1×106 per well with RPMI-1640 and supplemented with 1% heat inactivated human AB serum, 1% gentamicin and 0.25% PHA. Active reagents are added to each well after 24 h and pure medium formed the control for each substance. Cells are then harvested after a further three days[1].

动物实验

动物模型:ICR Swiss mice

化学信息

分子量

244.29

分子式

C15H16O3

CAS

484-12-8

溶解度

DMSO: 46 mg/mL (188.3 mM)

Ethanol: 46 mg/mL (188.3 mM)

Water: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

储存条件

store at -80°C

备注

For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

配制溶液

1 mg 5 mg 10 mg
1 mM 4.093 ml 20.467 ml 40.935 ml
5 mM 0.819 ml 4.093 ml 8.187 ml
10 mM 0.409 ml 2.047 ml 4.093 ml
50 mM 0.082 ml 0.409 ml 0.819 ml
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