Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Nitroxoline (5-nitroquinolin-8-ol) 是一种抗生素,防治生物膜感染。它通过从生物膜基质螯合Fe2+和Zn2+离子而发挥作用。
产品描述 | Nitroxoline (5-nitroquinolin-8-ol) is a urinary antibacterial agent active against susceptible gram-positive and gram-negative organisms commonly found in urinary tract infections. |
体外活性 | 使用分光荧光测定法,Nitroxoline可明显减少所有受测癌症细胞株细胞外 DQ 胶原 IV 的降解。Nitroxoline 还能明显减少实时监测到的肿瘤细胞侵袭,并减少多细胞肿瘤球体的侵袭生长,这种肿瘤球体可用作肿瘤侵袭的三维玻璃体模型。在体外血管生成试验中,Nitroxoline可显著减少内皮管的形成[1]。 |
体内活性 | Nitroxoline 在LPB纤维肉瘤和MMTV-PyMT乳腺癌小鼠模型中显著抑制了肿瘤生长、血管生成和转移[1]。 |
激酶实验 | In Vitro Kinase Assays : The potency of SP600125 towards kinases, including MPS1, JNK, and Aurora kinase A, is determined based on the specific measurement of radioactive phosphotransfer to the substrate. For each enzyme, the absolute Km values for ATP and the specific substrate are initially determined and each assay is then run at optimized [ATP] (2·αKm) and [substrate] (5·Km) concentrations. MPS1 activity is measured using 5 nM of MPS1 recombinant protein in 50 mM HEPES pH 7.5, 2.5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, 3 μM NaVO3, 2 mM β-glycerophosphate, 0.2 mg/mL BSA, 200 μM P38-βtide substrate-peptide (KRQADEEMTGYVATRWYRAE), and 8 μM ATP with 1.5 nM 33P-γ-ATP. Ten serial 1:3 dilutions (from 30 μM to 1.5 nM) of SP600125 are tested and IC50 determined. |
细胞实验 | cell suspension are seeded in the wells of an E-plate 16 according to the manufacturer's instructions. After seeding, the CI is monitored every 15 min. After ∼10 h (MCF-10A neoT and MMTV-PyMT), 14 h (U-87 MG) or 24 h (LPB), when the cells are in their log phase of growth, 50 μl of the compound or 0.1% DMSO is added, and the experiment allowed to run for 72 h. Once every 24 h the medium is replaced with fresh medium containing the inhibitor or suitable control to prevent cell death due to medium depletion. Compounds and their concentrations are: nitroxoline (5 μM) and CA-074 (5 μM) for all cell lines other than MCF-10A neoT cell line, where nitroxoline was used at 2.5 μM. All measurements were performed in quadruplicate. (Only for Reference) |
别名 | 5-Nitro-8-quinolinol, 8-Hydroxy-5-nitroquinoline, 5-Nitro-8-hydroxyquinoline, 5-nitroquinolin-8-ol, 硝羟喹啉 |
分子量 | 190.16 |
分子式 | C9H6N2O3 |
CAS No. | 4008-48-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 36 mg/mL (189.3 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 5.2587 mL | 26.2936 mL | 52.5873 mL | 131.4682 mL |
5 mM | 1.0517 mL | 5.2587 mL | 10.5175 mL | 26.2936 mL | |
10 mM | 0.5259 mL | 2.6294 mL | 5.2587 mL | 13.1468 mL | |
20 mM | 0.2629 mL | 1.3147 mL | 2.6294 mL | 6.5734 mL | |
50 mM | 0.1052 mL | 0.5259 mL | 1.0517 mL | 2.6294 mL | |
100 mM | 0.0526 mL | 0.2629 mL | 0.5259 mL | 1.3147 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Nitroxoline 4008-48-4 Autophagy Microbiology/Virology Antibacterial Antibiotic 5-Nitro-8-quinolinol Bacterial 8-Hydroxy-5-nitroquinoline 5-Nitro-8-hydroxyquinoline inhibit 5-nitroquinolin-8-ol Inhibitor 硝羟喹啉 inhibitor