store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Narciclasine (Lycoricidinol) 是一种植物生长调节剂。它通过调节Rho/Rho 激酶/LIM 激酶/cofilin 信号传导途径,大大增加GTP 酶RhoA 活性以及以RhoA 依赖性方式诱导肌动蛋白应力纤维形成。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 937 | 现货 | ||
5 mg | ¥ 3,950 | 现货 | ||
10 mg | ¥ 5,680 | 现货 | ||
25 mg | 待询 | 现货 | ||
50 mg | 待询 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 4,120 | 现货 |
产品描述 | Narciclasine (Lycoricidinol), a natural product, modulates the Rho/Rho-kinase/LIM kinase/cofilin signaling pathway, greatly increasing GTPase RhoA activity. |
体外活性 | Narciclasine在人脑胶质瘤多形性细胞中激活Rho和应力纤维(平均IC50约为50 nM,针对6种人脑胶质瘤多形性)。Narciclasine在一组60种癌细胞系中的平均IC50值为47 nM [1]。对于Narciclasine,其在根尖生长抑制的IC50测量值为0.1 μM [2]。 |
体内活性 | 静脉注射Narciclasine,剂量为每千克体重1毫克,显著提高了携带GL19恶性胶质瘤小鼠的存活率。以相同剂量,每周五次,连续五周口服Narciclasine也显著提高了该模型中动物的存活率。以每千克体重1毫克的剂量口服Narciclasine,显著提高了携带Hs683恶性胶质瘤小鼠的存活率。增加每周给药次数并未提高这些携带Hs683恶性胶质瘤小鼠的存活率[1]。 |
细胞实验 | The Narciclasine IC50 concentration, the Narciclasine concentration that decreased by 50% the global growth rate of a given cell population, is assessed with the MTT assay. The cells are incubated for 72 h in the presence and absence of the Narciclasine (with concentrations ranging between 1 and 10000 nM concentrate) for the determination of Narciclasine IC50 values [1]. |
动物实验 | The Hs683 cell line and GL19 primoculture grafted into the brains of nude immunodeficient mice both produced invasive brain tumors. Xenograft-bearing mice receive vehicle alone, oral temozolomide at 40 mg/kg (5 administrations per week for 5 consecutive weeks), or Narciclasine at 1 mg/kg either oral (once per week for 5 weeks) or i.v. (twice per week for 5 weeks). Drug administration is initiated respectively on days 5 and 7 post-tumor graftings for the Hs683 and GL19 models. The temozolomide dose and treatment schedule are selected based on previously optimized regimens. Narciclasine dose and treatment schedule are selected based on Narciclasine toxicity study in rats after oral administration and pharmacokinetic study that we have recently published. In toxicity study, Narciclasine (25, 10, or 1 mg/kg) is administered five times a week for 3 weeks and the no adverse effect level dose is defined to be 1 mg/kg/d p.o., with minimal acanthosis reactive changes and minor variations in some biochemistry parameters observed at this dose level considered to be nonadverse [1]. |
别名 | 水仙环素, Lycoricidinol |
分子量 | 307.26 |
分子式 | C14H13NO7 |
CAS No. | 29477-83-6 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 25 mg/mL (81.37 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.2546 mL | 16.2729 mL | 32.5457 mL | 81.3643 mL |
5 mM | 0.6509 mL | 3.2546 mL | 6.5091 mL | 16.2729 mL | |
10 mM | 0.3255 mL | 1.6273 mL | 3.2546 mL | 8.1364 mL | |
20 mM | 0.1627 mL | 0.8136 mL | 1.6273 mL | 4.0682 mL | |
50 mM | 0.0651 mL | 0.3255 mL | 0.6509 mL | 1.6273 mL |
中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
金花石蒜 | Lycoris aurea Herb | - | ― | |
石蒜 | Lycoris radiata(L’Her.) Herb., Lycoris chinensis Traub | 温 | 辛, 甘 | 肺, 胃, 肝 |
方剂名称 | 处方组成 | 剂型 | 处方来源 |
秘传妙济饮 | 石蒜 | 汤剂 | 《松崖医径》卷下。 |
痰核酒 | 都管草根,兔耳,石蒜,白果,紫花地丁,威灵仙 | 酒剂 | 《类证治裁》卷二。 |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Narciclasine 29477-83-6 Cell Cycle/Checkpoint Cytoskeletal Signaling Stem Cells ROCK Rho-associated kinase inhibit Inhibitor ROK 水仙环素 Lycoricidinol Rho-associated protein kinase Rho-kinase inhibitor