Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NVP-2 是一种具有选择性的 ATP 竞争性细胞周期蛋白依赖性激酶 9 探针,可抑制 CDK9/CycT 活性,IC50值为 0.514 nM。它对 CDK1/CycB,CDK2/CycA 和 CDK16/CycY 激酶具有抑制作用,可诱导细胞凋亡。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 497 | 现货 | ||
2 mg | ¥ 729 | 现货 | ||
5 mg | ¥ 1,220 | 现货 | ||
10 mg | ¥ 2,130 | 现货 | ||
25 mg | ¥ 3,590 | 现货 | ||
50 mg | ¥ 5,190 | 现货 | ||
100 mg | ¥ 7,370 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,380 | 现货 |
产品描述 | NVP-2 is an effective and selective ATP-competitive cyclin-dependent kinase 9 (CDK9) probe. NVP-2 induces cell apoptosis. NVP-2 inhibits CDK9/CycT activity (IC50: 0.514 nM). NVP-2 shows inhibitory effcts on CDK1/CycB, CDK2/CycA and CDK16/CycY kinases (IC50: 0.584 μM, 0.706 μM, and 0.605 μM, respectively). |
靶点活性 | CDK9:0.5 nM (IC50) |
体外活性 | NVP-2 has an anti-proliferation of leukemia cells, suppresses KOPT-K1, Jurkat, P12-ICHIKAWA, DU.528, MOLT 16, HSB-2, PF-382, SKW-3, SUP-T11, DND-41 and HPB-ALL cells with IC50 values of 0.1688 μM, 0.1233 μM,0.5736 μM,0.1575 μM, 0.1620 μM,0.1585 μM, 0.1808 μM, 0.2589 μM, 0.0918 μM and 0.3023 μM, respectively. NVP-2 (0-10 nM; 72 hours) shows CRBN-dependent anti-proliferative and pro-apoptotic effects in MOLT4 cells (IC50: 9 nM). NVP-2 (250 nM; 24 hours) causes cell apoptosis in MOLT4 cells, upregulates caspase-3 and γH2A.X expression. However, while the compound washout significantly reduces the degree of apoptosis induced by NVP-2. NVP-2 (250 nM-1 μM; 6 hours) engages CDK9 in wildtype and CRBN?/? MOLT4 cells at all concentrations, while CDK2 and CDK7 are unaffected. |
分子量 | 513.07 |
分子式 | C27H37ClN6O2 |
CAS No. | 1263373-43-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 100 mg/mL (194.91 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.9491 mL | 9.7453 mL | 19.4905 mL | 48.7263 mL |
5 mM | 0.3898 mL | 1.9491 mL | 3.8981 mL | 9.7453 mL | |
10 mM | 0.1949 mL | 0.9745 mL | 1.9491 mL | 4.8726 mL | |
20 mM | 0.0975 mL | 0.4873 mL | 0.9745 mL | 2.4363 mL | |
50 mM | 0.039 mL | 0.1949 mL | 0.3898 mL | 0.9745 mL | |
100 mM | 0.0195 mL | 0.0975 mL | 0.1949 mL | 0.4873 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NVP-2 1263373-43-8 Apoptosis Cell Cycle/Checkpoint CDK inhibit Cyclin dependent kinase NVP2 Inhibitor CRBN CDK9 Leukemia NVP 2 inhibitor