Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NSC-632839 (Ubiquitin Isopeptidase Inhibitor II) 是一种非选择性异肽酶抑制剂,可抑制USP2、USP7和SENP2,EC50分别为 45±4 μM、37±1 μM 和 9.8±1.8 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 178 | 现货 | ||
5 mg | ¥ 377 | 现货 | ||
10 mg | ¥ 569 | 现货 | ||
25 mg | ¥ 1,210 | 现货 | ||
50 mg | ¥ 1,950 | 现货 | ||
100 mg | ¥ 2,880 | 现货 | ||
500 mg | ¥ 6,590 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 377 | 现货 |
产品描述 | NSC-632839 (Ubiquitin Isopeptidase Inhibitor II) is a nonselective isopeptidase inhibitor, which inhibits USP2 (EC50: 45±4 μM), USP7 (EC50: 37±1 μM), and SENP2 (EC50: 9.8±1.8 μM). |
靶点活性 | USP7:37±1 μM(EC50), USP2:45±4 μM(EC50), SENP2:9.8±1.8 μM(EC50) |
体外活性 | NSC 632839 inhibits ubiquitin isopeptidases as illustrated by its ability to inhibit z-LRGG-AMC cleavage by crude lysates in the mid-micromolar range. NSC 632839 (1.2-150 μM) does not inhibit the PLA2, indicating that the inhibition for isopeptidases is selective. |
激酶实验 | In a 96-well-plate, 40 nM USP2, 40 nM USP7, or 20 nM SENP2 is preincubated with a concentration range of NSC 632839 (NCI/NIH developmental therapeutics program) or control for 30 min before supplementation with an equal volume of 60 nM Ub-PLA2/40 μM NBD C6-HPC (USP2 or 7) or 20 nM SUMO3-PLA2/40 μM NBD C6-HPC (SENP2). Relative activity of the enzymes is determined by measuring the RFU values at single time points within the initial linear range (USP, 50 min; USP7, 50 min; and SENP2, 30 min). The RFU values within the initial linear range are normalized such that isopeptidase+vehicle=0% inhibition and isopeptidase+NEM=100% inhibition. The EC50 values are determined as above. The inhibitory activity of the test compound against the reporter enzyme PLA2 is performed as described above except there is no preincubation step and the data are normalized such that free PLA2+vehicle=0% inhibition and free PLA2+EDTA=100% inhibition. PLA2 activity is determined 8 min after the addition of the reagents[1]. |
别名 | F6, Ubiquitin Isopeptidase Inhibitor II |
分子量 | 339.86 |
分子式 | C21H22ClNO |
CAS No. | 157654-67-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 6.25 mg/mL (18.39 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.9424 mL | 14.7119 mL | 29.4239 mL | 73.5597 mL |
5 mM | 0.5885 mL | 2.9424 mL | 5.8848 mL | 14.7119 mL | |
10 mM | 0.2942 mL | 1.4712 mL | 2.9424 mL | 7.356 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NSC632839 157654-67-6 Cell Cycle/Checkpoint DNA Damage/DNA Repair Ubiquitination DUB F 6 NSC-632839 inhibit F6 NSC 632839 Inhibitor DUBs Ubiquitin Isopeptidase Inhibitor II Deubiquitinase F-6 inhibitor