Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NSC59984 是 p53 通路激活剂,可诱导突变 p53 蛋白降解和 p73 活化。它通过 MDM2 和泛素-蛋白酶体途径而诱导突变型 p53 蛋白降解。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 283 | 现货 | ||
5 mg | ¥ 663 | 现货 | ||
10 mg | ¥ 1,120 | 现货 | ||
25 mg | ¥ 2,320 | 现货 | ||
50 mg | ¥ 3,730 | 现货 | ||
100 mg | ¥ 5,450 | 现货 | ||
500 mg | ¥ 11,300 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 728 | 现货 |
产品描述 | NSC59984, a p53 pathway activator, induces mutant p53 protein degradation and p73 activation. |
体外活性 | NSC59984 specifically restores p53 pathway signaling and induces p73-dependent cell death in mutant p53-expressing human colorectal cancer cells. NSC59984 also induces cell death in a panel of cancer cell lines bearing different p53 mutations with little or no genotoxicity in normal cells. [1] |
体内活性 | In nude mice bearing DLD-1 xenograft tumors, NSC59984 (45 mg/kg, i.p.) significantly represses tumor growth in a p73-dependent manner. [1] |
细胞实验 | Cells are seeded at 5,000 cells per well on 96-well plates. Cells are mixed with an equal volume of CellTiter-Glo reagents, following the manufacturer's protocol, and bioluminescence imaging is measured using the IVIS imager.(Only for Reference) |
分子量 | 265.27 |
分子式 | C12H15N3O4 |
CAS No. | 803647-40-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 49 mg/mL (184.7 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 49 mg/mL (184.7 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 3.7697 mL | 18.8487 mL | 37.6974 mL | 94.2436 mL |
5 mM | 0.7539 mL | 3.7697 mL | 7.5395 mL | 18.8487 mL | |
10 mM | 0.377 mL | 1.8849 mL | 3.7697 mL | 9.4244 mL | |
20 mM | 0.1885 mL | 0.9424 mL | 1.8849 mL | 4.7122 mL | |
50 mM | 0.0754 mL | 0.377 mL | 0.7539 mL | 1.8849 mL | |
100 mM | 0.0377 mL | 0.1885 mL | 0.377 mL | 0.9424 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NSC59984 803647-40-7 Apoptosis p53 inhibit Inhibitor NSC-59984 MDM-2/p53 NSC 59984 inhibitor