Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NSC23005 sodium 是新型 p18 抑制剂 (ED50=5.21 nM),在小鼠和人类模型中促进造血干细胞扩增。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 297 | 现货 | ||
2 mg | ¥ 437 | 现货 | ||
5 mg | ¥ 693 | 现货 | ||
10 mg | ¥ 996 | 现货 | ||
25 mg | ¥ 2,110 | 现货 | ||
50 mg | ¥ 3,190 | 现货 | ||
100 mg | ¥ 4,580 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 693 | 现货 |
产品描述 | NSC23005 sodium is a novel and effective p18 inhibitor (ED50=5.21 nM) in promoting Hematopoietic stem cells (HSCs) expansion in both murine and human models. |
靶点活性 | p18INK4C:5.21 nM (ED50) |
体外活性 | NSC23005 sodium is a novel class of INK4C (p18INK4C or p18) small molecule inhibitor (p18SMIs), which is initially found by in silico 3D screening. NSC23005 sodium shows the most potent bioactivity in hematopoietic stem cells (HSCs) expansion (ED50=5.21 nM). Notably, NSC23005 sodium does not show significant cytotoxicity toward 32D cells or HSCs, nor does it augment leukemia cell proliferation. NSC23005 sodium (ED50=5.21 nM), shows no activity in promoting the proliferation of leukemia cells. |
体内活性 | NSC23005 sodium selectively promote HSCs division by inhibiting p18, thereby activating CDK4/6. NSC23005 sodium is a novel and effective p18 inhibitor in promoting HSCs expansion in both murine and human models without significant cytotoxicity toward HSCs, nor did it augment leukemia cell proliferation. |
细胞实验 | NSC23005 sodium is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. c-Kit enriched bone marrow (BM) cells are cultured for 5 days with cytokine combination plus NSC23005 sodium or DMSO. As positive controls, primary uncultured bone marrow cells are treated by ultraviolet radiation (UV) for 10 minutes prior to the staining process for apoptosis analysis. Apoptosis and cell death are measured by AnnexinV and DAPI staining in the Annexin V-FITC Apoptosis Detection Kit. Apoptosis is measured on an FACS analyzer. The data is analyzed using FlowJo software[1]. |
分子量 | 305.32 |
分子式 | C13H16NNaO4S |
CAS No. | 1796596-46-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (196.52 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.2753 mL | 16.3763 mL | 32.7525 mL | 81.8813 mL |
5 mM | 0.6551 mL | 3.2753 mL | 6.5505 mL | 16.3763 mL | |
10 mM | 0.3275 mL | 1.6376 mL | 3.2753 mL | 8.1881 mL | |
20 mM | 0.1638 mL | 0.8188 mL | 1.6376 mL | 4.0941 mL | |
50 mM | 0.0655 mL | 0.3275 mL | 0.6551 mL | 1.6376 mL | |
100 mM | 0.0328 mL | 0.1638 mL | 0.3275 mL | 0.8188 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NSC23005 Sodium 1796596-46-7 Cell Cycle/Checkpoint CDK NSC23005 NSC 23005 NSC-23005 Sodium inhibit NSC-23005 Inhibitor inhibitor