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NSC-41589

产品编号 T2031 CAS 6310-41-4

别名: NSC41589

NSC-41589 是 N-[2-(methylsulfanyl) phenyl]acetamide。

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NSC-41589, CAS 6310-41-4

规格	价格/CNY	货期
1 mg	￥ 896	现货
2 mg	￥ 1,330	现货
5 mg	￥ 2,360	现货
10 mg	￥ 3,490	现货
25 mg	￥ 5,680	现货
50 mg	￥ 7,950	现货
100 mg	￥ 10,700	现货
1 mL * 10 mM (in DMSO)	￥ 2,590	现货

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纯度: 98.44%

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生物活性

化学信息

存储 & 溶解度

参考文献

产品描述

NSC-41589 is N-[2-(methylsulfanyl) phenyl]acetamide.

激酶实验

Direct PAI-I in vitro activity assays : The chromogenic assay is initiated by the addition of tiplaxtinin (10 – 100 μM final concentration, maximum DMSO concentration of 0.2%) to recombinant human PAI-1 (140 nM in pH 6.6 buffer). After a 15 minute incubation at 25°C, 70 nM of recombinant human t-PA is added, and the combination of tiplaxtinin, PAI-1 and tPA are incubated for an additional 30 minutes. After the second incubation, Spectrozyme tPA, is added and absorbance read at 405 nm at 0 and 60 minutes. Relative PAI-1 inhibitory activity is equal to the residual tPA activity in the tiplaxtinin / PAI-1 treatment. Control treatments include the complete inhibition of tPA by PAI-1 at the molar ratio employed (2:1), and the absence of any effect of the tiplaxtinin on t-PA alone. The immunofunctional assay is based upon the non-SDS dissociable interaction between tPA and active PAI-1. Assay plates are coated with 100 μl of a solution of t-PA (10 μg/ml in TBS), and kept at 4 °C overnight. Tiplaxtinin is dissolved in DMSO and diluted to a final concentration of 1-100 μM as described above. Tiplaxtinin is then incubated with human PAI-1 (50 ng/ml) for 15 minutes, and an aliquot of this solution added to the t-PA-coated plate for 1 h. The solution is aspirated from the plate, which is then washed with a buffer consisting of 0.05% Tween 20 and 0.1% BSA in TBS. This assay detects only active inhibitory PAI-1 (not latent or substrate) bound to the plate, and is quantitated using a monoclonal antibody against human PAI-1 (MA33B8). A 1000X dilution of MA33B8 is added to the plate and incubated at for one hour, aspirated and washed. A secondary antibody consisting of goat anti-mouse IgG (H+L)-AP alkaline phosphatase conjugate is added, incubated for one hour, aspirated and washed. A 100 μl aliquot of alkaline phosphatase solution is added, followed by determination of absorbance at 405 nm 60 minutes later.The quantitation of residual active PAI-1 bound to t-PA at varying concentrations of tiplaxtinin is used to determine the IC50 by fitting the results to a logistic dose-response program, with the IC50 defined as the concentration of compound required to achieve 50% inhibition of PAI-1 activity. The assay sensitivity is 5 ng/ml of human PAI-1 as determined from a standard curve ranging from 0-100 ng/ml of human PAI-1.

别名	NSC41589
分子量	181.25
分子式	C9H11NOS
CAS No.	6310-41-4

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 65 mg/mL (358.62 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.5172 mL	27.5862 mL	55.1724 mL	137.931 mL
	5 mM	1.1034 mL	5.5172 mL	11.0345 mL	27.5862 mL
	10 mM	0.5517 mL	2.7586 mL	5.5172 mL	13.7931 mL
	20 mM	0.2759 mL	1.3793 mL	2.7586 mL	6.8966 mL
	50 mM	0.1103 mL	0.5517 mL	1.1034 mL	2.7586 mL
	100 mM	0.0552 mL	0.2759 mL	0.5517 mL	1.3793 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

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容积 (起始)

浓度 (终)

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参考文献

1. Ishmaeva, E.A., Alimova, A.Z., Vereshchagina, Y.A. et al. Russ J Org Chem (2015) 51: 943. https://doi.org/10.1134/S107042801507009X

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

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