Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NSC 95397 是一种选择性的Cdc25双特异性磷酸酶抑制剂。它抑制丝裂原活化蛋白激酶磷酸酶-1(MKP-1),通过 MKP-1 和 ERK1/2 途径抑制结肠癌细胞的增殖并诱导细胞凋亡。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 413 | 现货 | ||
5 mg | ¥ 990 | 现货 | ||
10 mg | ¥ 1,490 | 现货 | ||
25 mg | ¥ 3,230 | 现货 | ||
50 mg | ¥ 4,780 | 现货 | ||
100 mg | ¥ 6,830 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 955 | 现货 |
产品描述 | NSC 95397 is a potent inhibitor of Cdc25 dual specificity phosphatase(Ki of 32, 96, and 40 nM for Cdc25A, Cdc25B, and Cdc25C, respectively). |
靶点活性 | CDC25A:32nM(ki), CDC25C:40 nM(ki), CDC25B:96nM(ki) |
体外活性 | NSC 95397 reduced cell viability and anchorage-independent growth of all the three colon cancer cell lines through inhibited proliferation and induced apoptosis via regulating cell-cycle-related proteins, including p21, cyclin-dependent kinases, and caspases[1]. |
细胞实验 | To measure the cell proliferation activity of NSC 95397 against colon cancer cells, SW480 (1 × 10^4), SW620 (2 × 10^4), and DLD-1 (1 × 10^4) cells were seeded into 96-well plates.?After overnight culture, NSC 95397 cells were added at the indicated concentrations.?After 24 h of incubation, cell proliferation was determined in vitro using a BrdU cell proliferation assay kit.?O.D. 450 values were analyzed by using a Multiskan PC.The apoptosis of colon cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD.?Briefly, cells were treated with the indicated concentration of NSC 95397 for 24 h. After 24 h, Cells were harvested and stained with PE Annexin V/7-AAD for 15 min. The stained cells were analyzed using FACSCanto II low cytometer and FCS Express software[1] |
分子量 | 310.39 |
分子式 | C14H14O4S2 |
CAS No. | 93718-83-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 100 mg/mL (322.18 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.2218 mL | 16.1088 mL | 32.2175 mL | 80.5438 mL |
5 mM | 0.6444 mL | 3.2218 mL | 6.4435 mL | 16.1088 mL | |
10 mM | 0.3222 mL | 1.6109 mL | 3.2218 mL | 8.0544 mL | |
20 mM | 0.1611 mL | 0.8054 mL | 1.6109 mL | 4.0272 mL | |
50 mM | 0.0644 mL | 0.3222 mL | 0.6444 mL | 1.6109 mL | |
100 mM | 0.0322 mL | 0.1611 mL | 0.3222 mL | 0.8054 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NSC 95397 93718-83-3 Apoptosis Metabolism Phosphatase NSC-95397 Inhibitor inhibit NSC95397 inhibitor