Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Monocrotaline (Crotaline) 是一种吡咯里西啶生物碱,从猪屎豆植物的种子中提取得到,可诱导啮齿动物产生肺动脉高血压。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 325 | 现货 | ||
50 mg | ¥ 622 | 现货 | ||
100 mg | ¥ 913 | 现货 | ||
200 mg | ¥ 1,660 | 现货 | ||
500 mg | ¥ 3,730 | 现货 | ||
1 g | ¥ 5,450 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 250 | 现货 |
产品描述 | Monocrotaline (Crotaline) is a pyrrolizidine alkaloid and a toxic plant constituent that poisons livestock and humans through the ingestion of contaminated grains and other foods. The alkaloid causes pulmonary artery hypertension, right ventricular hypertrophy, and pathological changes in the pulmonary vasculature. Significant attenuation of the cardiopulmonary changes is noted after oral magnesium treatment. |
体外活性 | Monocrotaline (MCT) is an 11-membered macrocyclic pyrrolizidine alkaloid (PA) derived from the seeds of the Crotalaria spectabilis plant[1]. Monocrotaline is a natural ligand that exhibits dose-dependent cytotoxicity with potent antineoplastic activity. The in vitro cytotoxicity of monocrotaline is proved at IC50 24.966 µg/mL and genotoxicity at 2 X IC50 against HepG2 cells[2]. |
体内活性 | MCT leads to a pulmonary vascular syndrome in rats characterized by proliferative pulmonary vasculitis, pulmonary hypertension (PH), and cor pulmonale[3]. Among preClinicalal models of pulmonary arterial hypertension (PAH), monocrotaline animal model offers the advantage of mimic several key aspects of human PAH, including vascular remodeling, proliferation of smooth muscle cells, endothelial dysfunction, upregulation of inflammatory cytokines, and right ventricle failure, requiring a single drug injection[4]. Changes in many pathways associated with the development of PH, including activated glycolysis, increased markers of proliferation, disruptions in carnitine homeostasis, increased inflammatory and fibrosis biomarkers, and a reduction in glutathione biosynthesis are observed with the injection of monocrotaline[5]. |
别名 | 野百合碱, Crotaline |
分子量 | 325.36 |
分子式 | C16H23NO6 |
CAS No. | 315-22-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/mL (169.04 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.0735 mL | 15.3676 mL | 30.7352 mL | 76.838 mL |
5 mM | 0.6147 mL | 3.0735 mL | 6.147 mL | 15.3676 mL | |
10 mM | 0.3074 mL | 1.5368 mL | 3.0735 mL | 7.6838 mL | |
20 mM | 0.1537 mL | 0.7684 mL | 1.5368 mL | 3.8419 mL | |
50 mM | 0.0615 mL | 0.3074 mL | 0.6147 mL | 1.5368 mL | |
100 mM | 0.0307 mL | 0.1537 mL | 0.3074 mL | 0.7684 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Monocrotaline 315-22-0 Stem Cells TGF-beta/Smad inhibit Inhibitor 野百合碱 Crotaline inhibitor