Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Miltefosine (HePC) 是一种广谱抗微生物剂,是治疗内脏和皮肤利什曼病的药物。它是 CTP 磷酸胆碱胞苷基转移酶的抑制剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 359 | 现货 | ||
100 mg | ¥ 454 | 现货 | ||
200 mg | ¥ 698 | 现货 | ||
500 mg | ¥ 938 | 现货 |
产品描述 | Miltefosine (HePC) is the treatment of visceral and cutaneous leishmaniasis drug , and is proceeding clinical trials for this in several countries. Several medical agents produce some potency against visceral or cutaneous leishmaniasis, however a 2005 survey concluded that miltefosine is the only effective oral treatment for both forms of leishmaniasis. |
靶点活性 | PKC:7 μM |
激酶实验 | Levels of enzymatically active caspase-3 are quantified using the ApoAlert Caspase Fluorescent assay kit. Briefly, 1×106 BC-1 PEL cells are treated with 50 μM Miltefosine, 50 μM Perifosine, or 20 nM NVP-BEZ235, as well as the respective vehicle controls. Cells are harvested and lysed 12 hours later. Equivalent micrograms of cell lysate for all samples are incubated with a fluorogenic caspase-3 substrate (DEVD-AFC). Cleavage of DEVD by caspase-3 releases AFC, the fluorescence of which is measured using a FLUOstar OPTIMA fluorometer, with excitation and emission filter wavelengths set to 400 and 505 nm, respectively[3]. |
细胞实验 | 2 × 105 PEL cells are treated with the therapeutic compounds at the indicated doses or with appropriate vehicle as a negative control. Cells are followed for 96 hours, and cell viability is determined by trypan blue exclusion performed in quadruplicate. (Only for Reference) |
别名 | HePC, 米替福新, Hexadecyl phosphocholine |
分子量 | 407.57 |
分子式 | C21H46NO4P |
CAS No. | 58066-85-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 10 mM
DMSO: Insoluble
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 2.4536 mL | 12.2678 mL | 24.5357 mL | 61.3392 mL |
5 mM | 0.4907 mL | 2.4536 mL | 4.9071 mL | 12.2678 mL | |
10 mM | 0.2454 mL | 1.2268 mL | 2.4536 mL | 6.1339 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Miltefosine 58066-85-6 Chromatin/Epigenetic Cytoskeletal Signaling Microbiology/Virology PI3K/Akt/mTOR signaling Proteases/Proteasome Akt HIV Protease PKC HePC PKB inhibit HIV Inhibitor Protein kinase B 米替福新 Hexadecyl phosphocholine Human immunodeficiency virus inhibitor