Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Methysticin, a major kavalactone extracted from kava, can induce CYP1A1.
产品描述 | Methysticin, a major kavalactone extracted from kava, can induce CYP1A1. |
体外活性 | Methysticin triggers the most profound inducing effect on CYP1A1. Methysticin is able to activate the AhR signaling pathway. Hepa1c1c7 cells are treated with various concentrations of kava extract (0-50 μg/mL) and six kavalactones (0-100 μM) for 24 h. The results indicate that kava extract at concentrations up to 50 μg/mL and kavalactones up to 100 μM do not induce cell death. For the following studies, kava extract at 0.78-6.25 μg/mL and kavalactones at 0.78-25 μM, concentrations that cause no damage to cells, are used [1]. |
体内活性 | Methysticin (6 mg/kg) is administered once a week for a period of 6 months to 6-month-old transgenic APP/Psen1 mice by oral gavage. Methysticin treatment activates the Nrf2 pathway in the hippocampus and cortex of mice. The Aβ deposition in brains of Methysticin-treated APP/Psen1 mice is not altered compared to untreated mice. However, Methysticin treatment significantly reduces microgliosis, astrogliosis, and secretion of the pro-inflammatory cytokines TNF-α and IL-17A. Methysticin treatment results in significant activation of the Nrf2/ARE pathway in the hippocampus and the cortex but not in the midbrain and cerebellum of ARE-luciferase reporter gene mice. Methysticin treatment significantly increases the expression of both genes compared to untreated animals [2]. |
别名 | DL-Methysticin, 麻醉椒苦素, (±)-Methystici |
分子量 | 274.27 |
分子式 | C15H14O5 |
CAS No. | 20697-20-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 1 mg/mL (3.65 mM)
DMSO: 5 mg/mL (18.23 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 3.646 mL | 18.2302 mL | 36.4604 mL | 91.1511 mL |
DMSO | 5 mM | 0.7292 mL | 3.646 mL | 7.2921 mL | 18.2302 mL |
10 mM | 0.3646 mL | 1.823 mL | 3.646 mL | 9.1151 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Methysticin 20697-20-5 Metabolism P450 Methystici DL-Methysticin 麻醉椒苦素 (±)-Methystici Inhibitor inhibitor inhibit