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Methylene Blue

Methylene Blue

产品编号 T6888   CAS 61-73-4
别名: 亚甲蓝, methylthioninium chloride, CI-52015, Tetramethylthionine chloride, 亚甲兰, Basic Blue 9

Methylene Blue (Tetramethylthionine chloride) 是鸟苷酸环化酶,是单胺氧化酶 A 和 NO 合酶抑制剂。它有抗伤害感受、抗疟疾、抗抑郁和抗焦虑作用,可研究高铁血红蛋白血症、神经退行性疾病和异环磷酰胺引起的脑病。它是血管加压剂,用作色素内窥镜检查中的染料。

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Methylene Blue Chemical Structure
Methylene Blue, CAS 61-73-4
规格 价格/CNY 货期 数量
50 mg ¥ 239 现货
100 mg ¥ 418 现货
500 mg ¥ 713 现货
1 mL * 10 mM (in DMSO) ¥ 164 现货
其他形式的 Methylene Blue:
产品目录号及名称: Methylene Blue (T6888)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Methylene Blue (Tetramethylthionine chloride) (IC50 of 1.9 μM) inhibits tau filament formation. And it inhibits soluble guanylyl cyclase. It is also used as a dye in chromoendoscopy.
靶点活性 Tau aggregation:1.9 μM, guanylate cyclase:
体外活性 MB could inactivate nitric oxide directly by generating superoxide anions, it may inhibit the action of nitric oxide synthase itself. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, there are MB directly activating calcium-dependent potassium channels and enhancing the release of noradrenaline from intracellular stores.
体内活性 Methylene Blue decreases C-GMP and vascular smooth muscle relaxation by inhibiting guanylate cyclase. Pharmacokinetic studies in rodents showed that MB injects by intravascular resulting in a rapid and extensive accumulation of this drug in the central nervous system (CNS). When MB is injected intravenously, it selectively gathers in parathyroid glands, therefore is easy for the identification of structures during surgery.
激酶实验 Isolated Integrin Binding Assays.:Purified integrin (1 μg/mL; 4℃) is used to coat 96-well microtitre plates, which are then blocked with bovine serum albumin (BSA) (3% in 1 mM CaCl2, 1 mM MgCl2,10 pM MnCl2, 100 mM NaC1,50 mM Tris-hydroxymethyl-aminomethane; pH 7.4), and incubated (3 h at 30 ℃) with biotinylated ligands (1 pg/mL in binding buffer: 0.1% BSA, 1 mM CaCl2,1 mM MgCl2, 10 μM MnCl2, 100 mM NaCl, 50 mM Tris-hydroxymethyl-aminomethane; pH 7.4) in the presence or absence of serially diluted peptides. After washing (3×5 min with binding buffer), the bound biotinylated ligand is detected with alkaline-phosphatase conjugated goat anti-biotin antibodies (1 μg/mL; 1 h, 37℃), using p-nitrophenyl phosphate as chromogen. Cyclo (-RGDfK) binding in the absence of competitor is defined as 100% signal; binding to blocked wells in the absence of integrin is defined as 0%. Concentrations of Cyclo (-RGDfK) required for 50% inhibition of signal (IC50 values) are estimated graphically.
细胞实验 Immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are exposed at the sixth day in vitro to MB (10 μM) for 2h. Assess the survival and dendritic arbor architecture of these cells 48h after this treatment paradigm.
动物实验 MB (5 or 50 mg/kg) diluted in normal saline were injected intraarterially into Sprague- Dawley male rats over the 30s.
别名 亚甲蓝, methylthioninium chloride, CI-52015, Tetramethylthionine chloride, 亚甲兰, Basic Blue 9
分子量 319.85
分子式 C16H18ClN3S
CAS No. 61-73-4

存储

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: 3.2 mg/mL (10 mM)

DMSO: 3.2 mg/mL (10 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.1265 mL 15.6323 mL 31.2647 mL 78.1616 mL
5 mM 0.6253 mL 3.1265 mL 6.2529 mL 15.6323 mL
10 mM 0.3126 mL 1.5632 mL 3.1265 mL 7.8162 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Vutskits L, et al. Anesthesiology. 108(4):684-692. 2. Hochgräfe K, et al. Acta Neuropathol Commun. 2015 May 10;3:25. 3. Masaki E, et al. Anesth Analg. 1999, 89(2):484-489. 4. Ginimuge PR, et al. J Anaesthesiol Clin Pharmacol. 2010, 26(4):517-520. 5. Fan Z, Tian Y, Chen Z, et al. Blocking interaction between SHP2 and PD‐1 denotes a novel opportunity for developing PD‐1 inhibitors[J]. EMBO Molecular Medicine. 2020: e11571.

文献引用

1. Fan Z, Tian Y, Chen Z, et al. Blocking interaction between SHP2 and PD‐1 denotes a novel opportunity for developing PD‐1 inhibitors. EMBO Molecular Medicine. 2020: e11571
Tirucallol Ginsenoside Rb3 anemarsaponin B Eburicoic acid Epibetulinic acid Chondroitin sulfate 4-Hydroxyflavanone Siegeskaurolic acid

相关化合物库

该产品包含在如下化合物库中:
抗癌临床化合物库 抗癌药物库 神经退行性疾病化合物库 抗寄生虫库 抗癌上市药物库 微管靶向化合物库 抗肺癌化合物库 神经信号分子库 细胞骨架化合物库 抗感染化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Methylene Blue 61-73-4 Cytoskeletal Signaling GPCR/G Protein Immunology/Inflammation Microbiology/Virology Neuroscience Guanylate cyclase Microtubule Associated NO Synthase Parasite Monoamine Oxidase antimalarial anxiolytic 亚甲蓝 inhibit Methylthioninium methemoglobinemias cGMP CI 52015 Nitric oxide synthases methylthioninium chloride NOS Tetramethylthionine Chloride Inhibitor antinociception CI-52015 CI52015 Tetramethylthionine chloride antidepressant dye methylthioninium Chloride MAO vasopressor Basic Blue-9 Guanylate Cyclase Basic Blue9 亚甲兰 Basic Blue 9 inhibitor

 

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