Powder: -20°C for 3 years | In solvent: -80°C for 1 year
MS049 2HCl (1502816-23-0(free base)) 是一种有效的选择性 PRMT4 (IC50 = 34 nM) 和 PRMT6 (IC50 = 43 nM) 抑制剂。它对其他 I 型 PRMT 的活性较低(对于 PRMT1、PRMT3 和 PRMT8,IC50分别<130、<220 和 1.6 μM,),并且对 II 型或 III 型 PRMT 没有抑制作用,也没有对任何其他甲基转移酶或非表观遗传靶标进行测试。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 280 | 待询 | ||
5 mg | ¥ 460 | 待询 | ||
10 mg | ¥ 750 | 待询 | ||
25 mg | ¥ 1,590 | 待询 | ||
50 mg | ¥ 2,990 | 待询 | ||
1 mL * 10 mM (in DMSO) | ¥ 460 | 待询 |
MS049 2HCl (1502816-23-0(free base)) 的其他形式现货产品:
产品描述 | MS049 is a potent and selective inhibitor of PRMT4 (IC50 = 34 nM) and PRMT6 (IC50 = 43 nM). It is less active against additional type I PRMTs (IC50s = >130, >220, and 1.6 μM for PRMT1, PRMT3, and PRMT8, respectively) and displays no inhibition against type II or type III PRMTs nor any additional methyltransferases or nonepigenetic targets tested. |
靶点活性 | PRMT4:34 nM, PRMT6:43 nM |
体外活性 | MS049 has been shown to reduce the H3R2me2a mark in HEK293 cells with an IC50 value of 0.97 μM and also, unexpectedly, to reduce H4R3me2a in HEK293 cells. |
细胞实验 | HEK293 cells were grown in 12-well plates in DMEM supplemented with 10% FBS, penicillin (100 units/mL) and streptomycin (100 μg/mL). 30% confluent cells were treated with different concentrations of compounds in triplicates or DMSO control for 72 h. Cells were lysed in 100 μL of total lysis buffer (20 mM Tris-HCl pH 8, 150 mM NaCl, 1 mM EDTA, 10 mM MgCl2, 0.5% TritonX-100, 12.5 U/mL benzonase, complete EDTA-free protease inhibitor cocktail ). After 3 min incubation at RT, SDS was added to final 1% concentration. Lysates were run on SDS-PAGE and immunoblotting was done as outlined below to determine Med12-Rme2a levels in Western blot. They are for reference only. |
分子量 | 321.28 |
分子式 | C15H26Cl2N2O |
CAS No. | T4393 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PBS(pH7.2): 10 mg/ml
DMSO: 30 mg/mL
Ethanol: 30 mg/mL
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
MS049 2HCl (1502816-23-0(free base)) T4393 Chromatin/Epigenetic Histone Methyltransferase MS049 2HCl (1502816230(free base)) MS049 2HCl (1502816 23 0(free base)) MS-049 2HCl (1502816-23-0(free base)) Inhibitor inhibitor inhibit