Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ML264 (CID-51003603) 是抗肿瘤剂,能够特异性地抑制 Krüppel 样因子 5 表达。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 289 | 现货 | ||
5 mg | ¥ 678 | 现货 | ||
10 mg | ¥ 980 | 现货 | ||
25 mg | ¥ 1,820 | 现货 | ||
50 mg | ¥ 2,970 | 现货 | ||
100 mg | ¥ 4,620 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 776 | 现货 |
产品描述 | ML264 (CID-51003603) is a selective kruppel-like factor 5 (KLF5) inhibitor, which potently Inhibits growth of Colorectal Cancer. |
靶点活性 | KLF5:29 nM |
体外活性 | ML264 potently halts DLD-1 viability (IC50 = 29 nM) with high maximal effect (>90%). DLD-1 cells are human colorectal adenocarcinoma cells. ML264 has significant effects at submicromolar doses on other cell types as well, including HCT116 (human colorectal carcinoma), HT29 (human colorectal adenocarcinoma), and SW620 (human colorectal adenocarcinoma). The IEC-6 anti-target (a nontransformed rat intestinal epithelial cell line) is largely unaffected, with inhibition below 50% at the highest dose[1].This compound potently inhibits proliferation of CRC cells in vitro through modifications of the cell cycle profile[2]. |
体内活性 | In an established xenograft mouse model of colon cancer, ML264 efficiently inhibits growth of the tumor within five days of treatment. this effect is caused by a significant reduction in proliferation and that ML264 potently inhibits the expression of KLF5 and EGR1, a transcriptional activator of KLF5[2]. |
细胞实验 | For cell proliferation experiments, DLD-1 and HCT116 cells are treated with 10μM ML264 or with vehicle (DMSO). Live cells are collected at 24, 48 and 72 hours post treatment and their numbers are determined by counting using a Coulter counter; In MTS assay, DLD-1 and HCT116 cells are treated with 10μM ML264 or with vehicle (DMSO). After 24, 48, and 72 hours of incubations, 20 μL of MTS solution is added to each well and an analysis is performed according to the manufacturer's protocol. (Only for Reference) |
别名 | CID-51003603 |
分子量 | 384.88 |
分子式 | C17H21ClN2O4S |
CAS No. | 1550008-55-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 71 mg/mL (184.5 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.5982 mL | 12.9911 mL | 25.9821 mL | 64.9553 mL |
5 mM | 0.5196 mL | 2.5982 mL | 5.1964 mL | 12.9911 mL | |
10 mM | 0.2598 mL | 1.2991 mL | 2.5982 mL | 6.4955 mL | |
20 mM | 0.1299 mL | 0.6496 mL | 1.2991 mL | 3.2478 mL | |
50 mM | 0.052 mL | 0.2598 mL | 0.5196 mL | 1.2991 mL | |
100 mM | 0.026 mL | 0.1299 mL | 0.2598 mL | 0.6496 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ML264 1550008-55-3 Cell Cycle/Checkpoint DNA Damage/DNA Repair MAPK DNA/RNA Synthesis KLF ML-264 CID-51003603 Inhibitor Krüppel-like factor inhibit ML 264 CID 51003603 CID51003603 inhibitor