Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ML216 (CID-49852229) 是一种选择性、细胞渗透性的BLM 解旋酶抑制剂,具有抗肿瘤活性。它对BLMfull-length 和BLM636-1298的IC50分别为 2.98 μM 和 0.97 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 448 | 现货 | ||
10 mg | ¥ 823 | 现货 | ||
25 mg | ¥ 1,658 | 现货 | ||
50 mg | ¥ 2,515 | 现货 | ||
100 mg | ¥ 3,362 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 496 | 现货 |
产品描述 | ML216 (CID-49852229) is a small molecule inhibitor of BLM helicase (IC50: 1.8 μM). It showed 28-fold selective against the related helicases RECQ1, RECQ5, and E. coli UvrD (IC50s > 50 μM). |
靶点活性 | BLM:1.8 μM |
体外活性 | ML216 shows cell-based activity and can induce sister chromatid exchanges, enhance the toxicity of aphidicolin, and exert antiproliferative activity in cells expressing BLM, but not those lacking BLM [2]. |
细胞实验 | Cells were treated with BLM inhibitors at the indicated concentrations for up to 72 hr. At each time point, the WST-1 reagent (a pale tetrazolium derivative converted to an intensely-colored formazan product by the action of mitochondrial dehydrogenases) was added, and after a 4 hr incubation the plates were analyzed optically at 450 nm with a reference wavelength of 690 nm [2]. |
别名 | CID-49852229 |
分子量 | 383.32 |
分子式 | C15H9F4N5OS |
CAS No. | 1430213-30-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 5 mg/mL (13 mM), Sonication and heating are recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.6088 mL | 13.0439 mL | 26.0879 mL | 65.2197 mL |
5 mM | 0.5218 mL | 2.6088 mL | 5.2176 mL | 13.0439 mL | |
10 mM | 0.2609 mL | 1.3044 mL | 2.6088 mL | 6.522 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ML216 1430213-30-1 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis helicase ML 216 WRN DNA sister inhibit Anticancer CID-49852229 anti-proliferative CID 49852229 Inhibitor CID49852229 chromatid BLM unwinding toxicity ML-216 inhibitor