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ML-7 hydrochloride

产品编号 T3050 CAS 110448-33-4

别名: ML-7 HCl, ML-7盐酸盐

ML-7 hydrochloride (ML-7 HCl) 是一种可渗透细胞的、可逆的、有效的、ATP 竞争性的肌球蛋白轻链激酶(MLCK) 特异性抑制剂，IC50为300 nM。它还抑制平滑肌肌球蛋白轻链激酶、PKA 和 PKC。

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ML-7 hydrochloride, CAS 110448-33-4

规格	价格/CNY	货期
5 mg	￥ 241	现货
10 mg	￥ 433	现货
25 mg	￥ 868	现货
50 mg	￥ 1,580	现货
100 mg	￥ 2,980	现货
200 mg	￥ 4,290	现货
1 mL * 10 mM (in DMSO)	￥ 241	现货

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其他形式的 ML-7 hydrochloride:

ML 7
询价

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纯度: 99.61%

纯度: 99%

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生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

产品描述	ML-7 hydrochloride (ML-7 HCl) is a cell-permeable, reversible, effective, ATP-competitive, and specific inhibitor of myosin light chain kinase (Ki: 300 nM); also inhibits smooth-muscle myosin light chain kinase, PKA, and PKC.
靶点活性	PKC:42 μM, PKA:21 μM, MLCK:0.3 μM(Ki)
体外活性	Inhibition of MLCK by ML-7 induces activation of caspase-3 in adherent MCF-10A and MCF-10A Ras-transformed cells[2]. Its treatment results in a dose-dependent decrease in MLC20 phosphorylation and a corresponding increase in cell death of SMC(smooth muscle cells). The inhibitory effect of ML-7 on MLCK is highly selective. The Ki of ML-7 for MLCK is 0.3 μM, while its Ki for protein kinase A is 21 μM and for protein kinase C is 42 μM. Overexpressing Bcl-2 can protect cells against apoptosis induced by ML-7[4].
体内活性	ML7 is able to improve Vascular endothelial dysfunction(VED) and atherosclerosis(AS) by regulating the expression of the tight junction (TJ) proteins zona occludens (ZO)-1 and occludin via mechanisms involving MLCK and MLC phosphorylation in high-fat diet-fed rabbits. ML7 decreases the expression of MLCK and MLC phosphorylation in the arterial wall of rabbits fed a high-fat diet and reduces lipid deposition lesions in AS rabbits[1].
激酶实验	IKK-2 Assay: Recombinant human IKK-2 (residues 1-756) is expressed in baculovirus as an N-terminal GST-tagged fusion protein, and its activity is assessed using a time-resolved fluorescence resonance energy transfer assay. In brief, IKK-2 (5 nM final) diluted in assay buffer (50 mM HEPES, 10 mM MgCl2, 1 mM CHAPS, pH 7.4, with 1 mM DTT and 0.01% w/v BSA) is added to wells containing various concentrations of compound or dimethyl sulfoxide (DMSO) vehicle (3% final). The reaction is initiated by the addition of GST-IκBα substrate (25 nM final)/ATP (1 μM final), in a total volume of 30 μL. The reaction is incubated for 30 min at room temperature, then terminated by the addition of 15 μL of 50 mM EDTA. Detection reagent (15 μL) in buffer (100 mM HEPES, pH 7.4, 150 mM NaCl, and 0.1% w/v BSA) containing antiphosphoserine- IκBα-32/36 monoclonal antibody 12C2, labeled with W-1024 europium chelate, and an allophycocyanin-labeled anti-GST antibody is added, and the reaction is further incubated for 60 min at room temperature. The degree of phosphorylation of GST- IκBαis measured as a ratio of specific 665-nm energy transfer signal to reference europium 620-nm signal, using a Packard Discovery plate reader.
细胞实验	Cells are treated with vehicle alone, Blebbistatin, ML-7, ML-9, latrunculin A or Y-27632 in media without serum at varying concentrations and time points before being fixed or prepared for FACS analysis. Untreated cells are also serum starved for the duration of the inhibitor treatment. Stock solutions of each agent are made in DMSO (50 μM Blebbistatin, 5 mM LA), 50% ethanol (10 mM ML-7), 70% ethanol (20 mM ML-9) or water (20 mM Y-27632) and are maintained at -20°C.(Only for Reference)

别名	ML-7 HCl, ML-7盐酸盐
分子量	452.74
分子式	C15H18ClIN2O2S
CAS No.	110448-33-4

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 22.6 mg/mL (50 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2088 mL	11.0439 mL	22.0877 mL	55.2193 mL
	5 mM	0.4418 mL	2.2088 mL	4.4175 mL	11.0439 mL
	10 mM	0.2209 mL	1.1044 mL	2.2088 mL	5.5219 mL
	20 mM	0.1104 mL	0.5522 mL	1.1044 mL	2.761 mL
	50 mM	0.0442 mL	0.2209 mL	0.4418 mL	1.1044 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

溶液体积

质量

配液浓度

参考文献

1. Cheng X, et al. Mol Med Rep. 2015, 12(3):4109-16. 2. Connell LE, et al. J Cell Sci. 2006, 119(Pt 11):2269-81. 3. Saitoh M, et al. J Biol Chem. 1987, 262(16):7796-801. 4. Fazal F, et al. Mol Cell Biol. 2005, 25(14):6259-66. 5. Zhang H, Lin F, Huang J, et al. Anisotropic stiffness gradient-regulated mechanical guidance drives directional migration of cancer cells[J]. Acta Biomaterialia. 2020.

文献引用

1. Zhang H, Lin F, Huang J, et al. Anisotropic stiffness gradient-regulated mechanical guidance drives directional migration of cancer cells. Acta Biomaterialia. 2020 2. Sun L, Sun L, Li X, et al. A Novel Tigecycline Adjuvant ML-7 Reverses the Susceptibility of Tigecycline-Resistant Klebsiella pneumoniae. Frontiers in cellular and infection microbiology. 2022: 1341.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

ML-7 hydrochloride 110448-33-4 Cell Cycle/Checkpoint Chromatin/Epigenetic Cytoskeletal Signaling Metabolism Stem Cells Tyrosine Kinase/Adaptors Serine/threonin kinase Myosin YAP PKA PKC ML-7 Hydrochloride Inhibitor Yes-associated protein ML-7 HCl inhibit ML-7 ML7 hydrochloride ML-7盐酸盐 ML7 ML7 Hydrochloride ML 7 ML 7 hydrochloride ML 7 Hydrochloride inhibitor

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ML-7 hydrochloride

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

文献引用

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords