Powder: -20°C for 3 years | In solvent: -80°C for 1 year
LY303511 是 LY294002 的结构类似物,可增强 SHEP-1 神经母细胞瘤细胞的TRAIL 敏感性,还可逆地阻断 MIN6 胰岛瘤细胞中的K+电流,IC50为64.6±9.1 μM。 在相同实验条件下,摩尔浓度相同的化合物盐形式与游离态具有相同的生物活性,但盐形式 LY 303511 hydrochloride 的水溶性和稳定性通常比游离态更好。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 4,530 | 6-8周 | ||
50 mg | ¥ 5,886 | 6-8周 | ||
100 mg | ¥ 8,240 | 6-8周 | ||
1 mL * 10 mM (in DMSO) | ¥ 850 | 6-8周 |
LY 303511 的其他形式现货产品:
产品描述 | LY303511, an inactive analogue of LY294002, is a mTOR inhibitor and no inhibition for PI3-K. |
靶点活性 | K+ current (MIN6 insulinoma cells):64.6±9.1 µM (IC50) |
激酶实验 | Mammalian DNA-PK (500 ng/μL) is isolated from HeLa cell nuclear extract after chromatography using Q-Sepharose, S-Sepharose, and Heparin agarose. DNA-PK (250 ng) activity is measured at 30°C, in a final volume of 40 μL, in buffer containing 25 mM HEPES (pH 7.4), 12.5 mM MgCl2, 50 mM KCl, 1 mM DTT, 10% v/v Glycerol, 0.1% w/v NP-40, and 1 mg of the substrate GST-p53N66 (the NH2-terminal 66 amino acid residues of human wild-type p53 fused to glutathione S-transferase) in polypropylene 96-well plates. To the assay mix, varying concentrations of inhibitor (in DMSO at a final concentration of 1% v/v) are added. After 10 min of incubation, ATP is added to give a final concentration of 50 μM, along with a 30-mer double-stranded DNA oligonucleotide (final concentration of 0.5 ng/mL), to initiate the reaction. After 1 h with shaking, 150 μL of PBS are added to the reaction, and 5 μL are then transferred to a 96-well opaque white plate containing 45 μL of PBS per well, where the GSTp53N66 substrate is allowed to bind to the wells for 1 h. To detect the phosphorylation event on the serine 15 residue of p53 elicited by DNA-PK, a p53 phosphoserine-15 antibody is used in a basic ELISA procedure. An antirabbit horseradish peroxidase-conjugated secondary antibody is then used in the ELISA before the addition of chemiluminescence reagent to detect the signal as measured by chemiluminescent counting via a TopCount NXT[1]. |
分子量 | 306.36 |
分子式 | C19H18N2O2 |
CAS No. | 154447-38-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 100 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.2641 mL | 16.3207 mL | 32.6413 mL | 81.6033 mL |
5 mM | 0.6528 mL | 3.2641 mL | 6.5283 mL | 16.3207 mL | |
10 mM | 0.3264 mL | 1.6321 mL | 3.2641 mL | 8.1603 mL | |
20 mM | 0.1632 mL | 0.816 mL | 1.6321 mL | 4.0802 mL | |
50 mM | 0.0653 mL | 0.3264 mL | 0.6528 mL | 1.6321 mL | |
100 mM | 0.0326 mL | 0.1632 mL | 0.3264 mL | 0.816 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
LY 303511 154447-38-8 Apoptosis Membrane transporter/Ion channel PI3K/Akt/mTOR signaling Potassium Channel TNF mTOR KcsA Inhibitor Tumor Necrosis Factor Receptor LY-303511 inhibit TNFR LY303511 TNF Receptor inhibitor