Powder: -20°C for 3 years | In solvent: -80°C for 1 year
LMK-235 是一种有效的 HDAC 抑制剂,可用于癌症研究。它可抑制 HDAC1、HDAC2、HDAC4、HDAC5、HDAC6、HDAC11 和 HDAC8 的活性,IC50值分别为 320 nM、881 nM、11.9 nM、4.22 nM、55.7 nM、852 nM 和 1278 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 185 | 现货 | ||
5 mg | ¥ 396 | 现货 | ||
10 mg | ¥ 617 | 现货 | ||
25 mg | ¥ 1,190 | 现货 | ||
50 mg | ¥ 1,980 | 现货 | ||
100 mg | ¥ 3,730 | 现货 | ||
200 mg | ¥ 5,420 | 现货 | ||
500 mg | ¥ 8,380 | 待询 | ||
1 mL * 10 mM (in DMSO) | ¥ 398 | 现货 |
产品描述 | LMK-235 is a potent HDAC inhibitor, and is used in cancer research. |
靶点活性 | HDAC5:4.2 nM, HDAC4:11.9 nM |
体内活性 | LMK-235对乳腺肿瘤细胞系MDA-MB-231,舌癌细胞系Cal27和食道癌细胞系Kyse510表现出高细胞毒性,并明显增强顺铂的细胞毒性。此外,LMK-235也对多种疟原虫生命周期阶段表现出纳摩尔级的活性。LMK-235在对顺铂化合物敏感性不同的人癌细胞系中引起HDAC抑制,IC50 <1 μM。 |
激酶实验 | HDAC IC50 Profiling: The in vitro inhibitory activity of compounds against seven human HDAC isoforms (1, 2, 4 C2A, 5 C2A, 6, 8, and 11) are performed with a fluorescent based assay according to the company's standard operating procedure. The IC50 values are determined using 10 different concentrations with 3-fold serial dilution starting at 10 μM. TSA and vorinostat are used as reference compounds. |
细胞实验 | The rate of cell survival under the action of test substances is evaluated by an improved MTT assay. The assay is based on the ability of viable cells to metabolize yellow MTT to violet formazan that can be detected spectrophotometrically. In brief, A2780, Cal27, Kyse510, and MDA-MB-231 cell lines are seeded at a density of 5000, 7000, 8000, and 10 000 cells/well in 96-well plates. After 24 h, cells are exposed to increased concentrations of the test compounds. Incubation is ended after 72 h, and cell survival is determined by addition of MTT solution (5 mg/mL in phosphate buffered saline). The formazan precipitate is dissolved in DMSO. Absorbance s measured at 544 and 690 nm in a FLUOstar microplate reader. (Only for Reference) |
别名 | LMK235 |
分子量 | 294.35 |
分子式 | C15H22N2O4 |
CAS No. | 1418033-25-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 29.4 mg/mL (100 mM)
Ethanol: 29.4 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.3973 mL | 16.9866 mL | 33.9732 mL | 84.9329 mL |
5 mM | 0.6795 mL | 3.3973 mL | 6.7946 mL | 16.9866 mL | |
10 mM | 0.3397 mL | 1.6987 mL | 3.3973 mL | 8.4933 mL | |
20 mM | 0.1699 mL | 0.8493 mL | 1.6987 mL | 4.2466 mL | |
50 mM | 0.0679 mL | 0.3397 mL | 0.6795 mL | 1.6987 mL | |
100 mM | 0.034 mL | 0.1699 mL | 0.3397 mL | 0.8493 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
LMK-235 1418033-25-6 Chromatin/Epigenetic DNA Damage/DNA Repair HDAC LMK 235 Histone deacetylases LMK235 Inhibitor inhibit inhibitor