Powder: -20°C for 3 years | In solvent: -80°C for 1 year
LLY-507 是一种有效的、细胞活性的、特异性的蛋白质赖氨酸甲基转移酶 SMYD2 抑制剂。它是一种化学探针,用于解析 SMYD2 在癌症和其他生物过程中的功能。它有抑制 SMYD2 甲基化 p53 肽的能力,IC50小于 15 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 297 | 现货 | ||
2 mg | ¥ 442 | 现货 | ||
5 mg | ¥ 722 | 现货 | ||
10 mg | ¥ 1,250 | 现货 | ||
25 mg | ¥ 2,890 | 现货 | ||
50 mg | ¥ 4,360 | 现货 | ||
100 mg | ¥ 6,180 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 913 | 现货 |
产品描述 | LLY-507 is an effective, cell-active, and specific inhibitor of protein-lysine Methyltransferase SMYD2. |
靶点活性 | SMYD2:15 nM. |
体外活性 | LLY-507 effectively inhibits the ability of SMYD2 to methylate p53 peptide (IC50<15 nM). LLY-507 is able to potently inhibit the methylation of the H4 peptide by the SMYD2 enzyme (IC50: 31 nM). It has 100-fold selectivity for SMYD2 than 24 other protein or DNA methyltransferases including related family members SMYD3 and SUVH420H1/SUV420H2. LLY-507 inactive (>20 μM) against three cytochrome P450 enzymes, 14 nuclear hormone receptors, 35 G protein-coupled receptors, and 454 kinases. LLY-507 dose-dependently inhibits the proliferation of several liver, esophageal, and breast cancer cell lines. |
细胞实验 | To examine the methylation status of p53 in HEK293 cells treated with LLY-507 by Western blotting, 2 × 105 cells are seeded in 6-well plates in triplicate and co-transfected with FLAG-tagged p53 and FLAG-tagged SMYD2 using Lipofectamine? 2000. The day after transfection, cells are treated with 0-2.5 μM LLY-507 for 28 h, then collected, and lysed in RIPA buffer. Cell lysates are subject to 10% SDS-PAGE and transferred to a PVDF membrane. |
别名 | LLY507, LLY 507 |
分子量 | 574.76 |
分子式 | C36H42N6O |
CAS No. | 1793053-37-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 93 mg/mL (161.8 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 21 mg/mL (36.5 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 1.7399 mL | 8.6993 mL | 17.3986 mL | 43.4964 mL |
5 mM | 0.348 mL | 1.7399 mL | 3.4797 mL | 8.6993 mL | |
10 mM | 0.174 mL | 0.8699 mL | 1.7399 mL | 4.3496 mL | |
20 mM | 0.087 mL | 0.435 mL | 0.8699 mL | 2.1748 mL | |
DMSO | 50 mM | 0.0348 mL | 0.174 mL | 0.348 mL | 0.8699 mL |
100 mM | 0.0174 mL | 0.087 mL | 0.174 mL | 0.435 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
LLY-507 1793053-37-8 Chromatin/Epigenetic Histone Methyltransferase protein LLY507 inhibit methyltransferase methylation breast cancer LLY 507 SMYD2 p53 Inhibitor epigenetics inhibitor