Powder: -20°C for 3 years | In solvent: -80°C for 1 year
L-Canavanine sulfate 是一种诱导型 NO 合酶的选择性抑制剂。
产品描述 | L-Canavanine sulfate is a selective inducible NO synthase inhibitor. |
体外活性 | L-Canavanine sulfate,which is hardly toxic alone, potentiated the cytotoxicity of VIN and PTX in HeLa and hepatocellular carcinoma cells[1]. |
体内活性 | Intravenous infusion of L-canavanine (100 mg/kg), a selective inhibitor of inducible NO synthase in vitro, 60 min and 180 min after lipopolysaccharide challenge, produced an increase in mean arterial pressure and reversed the lipopolysaccharide induced hypotension. However, in lipopolysaccharide challenged animals protected from hypotension by administration of L-canavanine (60 min post challenge), intravenous infusion of NG-nitro-L-arginine methyl ester at 180 min post challenge caused an immediate rise in mean arterial pressure, followed by a rapid fall in blood pressure and heart rate, and sudden death. In contrast, a second dose of L-canavanine at 180 min post challenge maintained blood pressure for the duration of the experiment. Inhibition of both constitutive and inducible NO synthase during endotoxaemia is lethal[2]. |
细胞实验 | The following cancer cells grown in arginine-rich and arginine-free media were employed: HeLa, Hep G2 and SK-HEP-1. Drug combination experiment used a method based on the median-effect principle and mass-action law[1]. |
分子量 | 274.25 |
分子式 | C5H14N4O7S |
CAS No. | 2219-31-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 100 mg/mL (364.63 mM), sonification/heating is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 3.6463 mL | 18.2315 mL | 36.4631 mL | 91.1577 mL |
5 mM | 0.7293 mL | 3.6463 mL | 7.2926 mL | 18.2315 mL | |
10 mM | 0.3646 mL | 1.8232 mL | 3.6463 mL | 9.1158 mL | |
20 mM | 0.1823 mL | 0.9116 mL | 1.8232 mL | 4.5579 mL | |
50 mM | 0.0729 mL | 0.3646 mL | 0.7293 mL | 1.8232 mL | |
100 mM | 0.0365 mL | 0.1823 mL | 0.3646 mL | 0.9116 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
L-Canavanine sulfate 2219-31-0 Immunology/Inflammation NF-Κb NO Synthase NOD Nitric oxide synthases Inhibitor inhibit L Canavanine sulfate LCanavanine sulfate L-Canavanine NOS L-Canavanine Sulfate inhibitor